NAD⁺依赖性异柠檬酸脱氢酶在棕色脂肪组织中的表达。
Expression of NAD(+)-dependent isocitrate dehydrogenase in brown adipose tissue.
作者信息
Shinohara Y, Daikoku T, Kajimoto K, Shima A, Yamazaki N, Terada H
机构信息
Faculty of Pharmaceutical Sciences, University of Tokushima, Shomachi-1, Tokushima, 770-8505, Japan.
出版信息
Biochem Biophys Res Commun. 2001 Mar 2;281(3):634-8. doi: 10.1006/bbrc.2001.4351.
cDNA clones significantly expressed in brown adipose tissue (BAT) but not in white adipose tissue (WAT) of rats were isolated by use of a PCR-select cDNA subtraction kit. Of the isolated clones, structural features of two of them, 2-58 and 2-67, were studied in detail. The results indicated that these clones were cDNAs encoding alpha- and beta-subunits of rat NAD(+)-dependent isocitrate dehydrogenase (NAD(+)-ICDH). Previous biochemical study suggested the importance of NAD(+)-ICDH in metabolism in BAT; however, transcript levels of individual subunits of this enzyme in BAT had never been analyzed. In the present study, using these newly isolated cDNAs, we clearly demonstrate that the expression of three subunits of NAD(+)-ICDH was the most remarkable in BAT among the various tissues analyzed.
利用PCR选择性cDNA扣除试剂盒，分离出在大鼠棕色脂肪组织（BAT）中显著表达但在白色脂肪组织（WAT）中不表达的cDNA克隆。在分离出的克隆中，对其中两个克隆2-58和2-67的结构特征进行了详细研究。结果表明，这些克隆是编码大鼠NAD(+)依赖异柠檬酸脱氢酶（NAD(+)-ICDH）α和β亚基的cDNA。先前的生化研究表明NAD(+)-ICDH在BAT代谢中很重要；然而，该酶各个亚基在BAT中的转录水平从未被分析过。在本研究中，使用这些新分离的cDNA，我们清楚地证明，在分析的各种组织中，NAD(+)-ICDH三个亚基的表达在BAT中最为显著。
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