错配结合蛋白MutS对含有不同错配的异源双链体的亲和力。
Affinity of mismatch-binding protein MutS for heteroduplexes containing different mismatches.
作者信息
Brown J, Brown T, Fox K R
机构信息
Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton S016 7PX, UK.
出版信息
Biochem J. 2001 Mar 15;354(Pt 3):627-33. doi: 10.1042/0264-6021:3540627.
Abstract
We have used bandshift analysis to measure the interaction between the Escherichia coli mismatch-binding protein MutS and synthetic DNA fragments containing all possible DNA mismatches as well as an unpaired T (DeltaT). The order of affinity is found to be DeltaT>GT>GG>AA approximately TT approximately TC>CA>GA>CC>GC. We find that the affinity for GT mismatches is affected by the flanking sequence and decreases in the order G(n)C(n)>(GC)(n)>A(n)T(n)>(AT)(n). Studies with base analogues show good binding to phiT (where phi represents 1',2'-dideoxyribose), but much weaker binding to Gphi.
摘要
我们利用凝胶迁移分析来测定大肠杆菌错配结合蛋白MutS与含有所有可能DNA错配以及一个未配对T（ΔT）的合成DNA片段之间的相互作用。发现亲和力顺序为ΔT>GT>GG>AA≈TT≈TC>CA>GA>CC>GC。我们发现，对GT错配的亲和力受侧翼序列影响，按G(n)C(n)>(GC)(n)>A(n)T(n)>(AT)(n)的顺序降低。使用碱基类似物的研究表明，其与ϕT（其中ϕ代表1',2'-二脱氧核糖）结合良好，但与Gϕ的结合则弱得多。
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