• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

错配结合蛋白MutS对含有不同错配的异源双链体的亲和力。

Affinity of mismatch-binding protein MutS for heteroduplexes containing different mismatches.

作者信息

Brown J, Brown T, Fox K R

机构信息

Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton S016 7PX, UK.

出版信息

Biochem J. 2001 Mar 15;354(Pt 3):627-33. doi: 10.1042/0264-6021:3540627.

DOI:10.1042/0264-6021:3540627
PMID:11237867
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1221694/
Abstract

We have used bandshift analysis to measure the interaction between the Escherichia coli mismatch-binding protein MutS and synthetic DNA fragments containing all possible DNA mismatches as well as an unpaired T (DeltaT). The order of affinity is found to be DeltaT>GT>GG>AA approximately TT approximately TC>CA>GA>CC>GC. We find that the affinity for GT mismatches is affected by the flanking sequence and decreases in the order G(n)C(n)>(GC)(n)>A(n)T(n)>(AT)(n). Studies with base analogues show good binding to phiT (where phi represents 1',2'-dideoxyribose), but much weaker binding to Gphi.

摘要

我们利用凝胶迁移分析来测定大肠杆菌错配结合蛋白MutS与含有所有可能DNA错配以及一个未配对T（ΔT）的合成DNA片段之间的相互作用。发现亲和力顺序为ΔT>GT>GG>AA≈TT≈TC>CA>GA>CC>GC。我们发现，对GT错配的亲和力受侧翼序列影响，按G(n)C(n)>(GC)(n)>A(n)T(n)>(AT)(n)的顺序降低。使用碱基类似物的研究表明，其与ϕT（其中ϕ代表1',2'-二脱氧核糖）结合良好，但与Gϕ的结合则弱得多。

相似文献

1
Affinity of mismatch-binding protein MutS for heteroduplexes containing different mismatches.错配结合蛋白MutS对含有不同错配的异源双链体的亲和力。
Biochem J. 2001 Mar 15;354(Pt 3):627-33. doi: 10.1042/0264-6021:3540627.
2
Identification of proteins of Escherichia coli and Saccharomyces cerevisiae that specifically bind to C/C mismatches in DNA.鉴定大肠杆菌和酿酒酵母中特异性结合DNA中C/C错配的蛋白质。
Nucleic Acids Res. 2000 Jul 1;28(13):2551-6. doi: 10.1093/nar/28.13.2551.
3
MutS interaction with mismatch and alkylated base containing DNA molecules detected by optical biosensor.通过光学生物传感器检测MutS与含有错配和烷基化碱基的DNA分子的相互作用。
Mutat Res. 1996 Nov 11;372(1):87-96. doi: 10.1016/S0027-5107(96)00170-4.
4
Disruption of the helix-u-turn-helix motif of MutS protein: loss of subunit dimerization, mismatch binding and ATP hydrolysis.MutS蛋白螺旋-转角-螺旋基序的破坏：亚基二聚化、错配结合及ATP水解功能丧失。
J Mol Biol. 2001 Jan 26;305(4):805-16. doi: 10.1006/jmbi.2000.4367.
5
MutS recognition: multiple mismatches and sequence context effects.MutS识别：多个错配及序列上下文效应
J Biosci. 2001 Dec;26(5):595-606. doi: 10.1007/BF02704758.
6
Binding specificities of the mismatch binding protein, MutS, to oligonucleotides containing modified bases.错配结合蛋白MutS与含修饰碱基的寡核苷酸的结合特异性。
Nucleic Acids Res Suppl. 2001(1):221-2. doi: 10.1093/nass/1.1.221.
7
The Escherichia coli MutL protein stimulates binding of Vsr and MutS to heteroduplex DNA.大肠杆菌MutL蛋白刺激Vsr和MutS与异源双链DNA的结合。
Nucleic Acids Res. 1998 Feb 15;26(4):948-53. doi: 10.1093/nar/26.4.948.
8
Mismatch-containing oligonucleotide duplexes bound by the E. coli mutS-encoded protein.由大肠杆菌mutS编码蛋白结合的含错配寡核苷酸双链体。
Nucleic Acids Res. 1988 Aug 25;16(16):7843-53. doi: 10.1093/nar/16.16.7843.
9
One tube mutation detection using sensitive fluorescent dyeing of MutS protected DNA.使用对MutS保护的DNA进行灵敏荧光染色的单管突变检测。
Nucleic Acids Res. 2000 Apr 15;28(8):E36. doi: 10.1093/nar/28.8.e36.
10
Binding of MutS mismatch repair protein to DNA containing UV photoproducts, "mismatched" opposite Watson--Crick and novel nucleotides, in different DNA sequence contexts.MutS错配修复蛋白与含有紫外线光产物的DNA的结合，这些光产物在不同的DNA序列背景下与沃森-克里克核苷酸和新核苷酸“错配”相对。
DNA Repair (Amst). 2005 Aug 15;4(9):983-93. doi: 10.1016/j.dnarep.2005.04.018.

引用本文的文献

1
DNA mutagenesis driven by transcription factor competition with mismatch repair.由转录因子与错配修复竞争驱动的DNA诱变。
Cell. 2025 Jul 23. doi: 10.1016/j.cell.2025.07.003.
2
Flipping the switch on some of the slowest mutating genomes: Direct measurements of plant mitochondrial and plastid mutation rates in msh1 mutants.开启一些突变速度最慢的基因组的开关：对msh1突变体中植物线粒体和质体突变率的直接测量。
PLoS Genet. 2025 Jun 30;21(6):e1011764. doi: 10.1371/journal.pgen.1011764. eCollection 2025 Jun.
3
A high-throughput single-molecule platform to study DNA supercoiling effect on protein-DNA interactions.一种用于研究DNA超螺旋对蛋白质-DNA相互作用影响的高通量单分子平台。
Nucleic Acids Res. 2025 Jun 20;53(12). doi: 10.1093/nar/gkaf581.
4
A high throughput single molecule platform to study DNA supercoiling effect on protein-DNA interactions.一种用于研究DNA超螺旋对蛋白质-DNA相互作用影响的高通量单分子平台。
bioRxiv. 2024 Oct 25:2024.10.24.620099. doi: 10.1101/2024.10.24.620099.
5
Adaptation delay causes a burst of mutations in bacteria responding to oxidative stress.适应延迟导致细菌对氧化应激反应产生突变爆发。
EMBO Rep. 2023 Jan 9;24(1):e55640. doi: 10.15252/embr.202255640. Epub 2022 Nov 17.
6
Serial enrichment of heteroduplex DNA using a MutS-magnetic bead system.利用 MutS 磁珠系统对异源双链 DNA 进行连续富集。
Biotechnol J. 2023 Jan;18(1):e2200323. doi: 10.1002/biot.202200323. Epub 2022 Nov 22.
7
Mismatch Repair: From Preserving Genome Stability to Enabling Mutation Studies in Real-Time Single Cells.错配修复：从保护基因组稳定性到实时单细胞突变研究。
Cells. 2021 Jun 18;10(6):1535. doi: 10.3390/cells10061535.
8
Melting temperature measurement and mesoscopic evaluation of single, double and triple DNA mismatches.单链、双链和三链DNA错配的解链温度测量及介观评估
Chem Sci. 2020 Jul 23;11(31):8273-8287. doi: 10.1039/d0sc01700k.
9
Control of Genome Stability by EndoMS/NucS-Mediated Non-Canonical Mismatch Repair.内溶酶体/核小体介导的非经典错配修复对基因组稳定性的控制。
Cells. 2021 May 25;10(6):1314. doi: 10.3390/cells10061314.
10
MutS protein-based fiber optic particle plasmon resonance biosensor for detecting single nucleotide polymorphisms.基于 MutS 蛋白的光纤颗粒等离子体共振生物传感器用于检测单核苷酸多态性。
Anal Bioanal Chem. 2021 May;413(12):3329-3337. doi: 10.1007/s00216-021-03271-1. Epub 2021 Mar 13.

本文引用的文献

1
Repair of defined single base-pair mismatches in Escherichia coli.大肠杆菌中定义的单个碱基对错配的修复。
Proc Natl Acad Sci U S A. 1985 Jan;82(2):503-5. doi: 10.1073/pnas.82.2.503.
2
Recognition of GT mismatches by Vsr mismatch endonuclease.Vsr错配核酸内切酶对GT错配的识别。
Nucleic Acids Res. 2000 Jul 1;28(13):2535-40. doi: 10.1093/nar/28.13.2535.
3
Modulation of MutS ATP hydrolysis by DNA cofactors.DNA辅助因子对MutS ATP水解的调节作用。
Biochemistry. 2000 Mar 21;39(11):3176-83. doi: 10.1021/bi992286u.
4
Biochemical characterization of the interaction between the Saccharomyces cerevisiae MSH2-MSH6 complex and mispaired bases in DNA.酿酒酵母MSH2-MSH6复合物与DNA中错配碱基之间相互作用的生化特性
J Biol Chem. 1999 Sep 17;274(38):26668-82. doi: 10.1074/jbc.274.38.26668.
5
Oligomerization of a MutS mismatch repair protein from Thermus aquaticus.嗜热水生栖热菌MutS错配修复蛋白的寡聚化
J Biol Chem. 1999 Aug 13;274(33):23673-8. doi: 10.1074/jbc.274.33.23673.
6
ATP hydrolysis-dependent formation of a dynamic ternary nucleoprotein complex with MutS and MutL.ATP水解依赖的与MutS和MutL形成动态三元核蛋白复合物。
Nucleic Acids Res. 1999 Jun 1;27(11):2325-31. doi: 10.1093/nar/27.11.2325.
7
Eukaryotic mismatch repair: an update.真核生物错配修复：最新进展
Mutat Res. 1998 Dec 14;409(3):107-21. doi: 10.1016/s0921-8777(98)00056-1.
8
Nucleotide-promoted release of hMutSalpha from heteroduplex DNA is consistent with an ATP-dependent translocation mechanism.核苷酸促进hMutSalpha从异源双链DNA上释放，这与ATP依赖的转位机制一致。
J Biol Chem. 1998 Nov 27;273(48):32055-62. doi: 10.1074/jbc.273.48.32055.
9
DNA-dependent activation of the hMutSalpha ATPase.hMutSα 腺苷三磷酸酶的DNA依赖性激活。
J Biol Chem. 1998 Nov 27;273(48):32049-54. doi: 10.1074/jbc.273.48.32049.
10
Replication errors: cha(lle)nging the genome.复制错误：挑战基因组。 （注：原文中“cha(lle)nging”拼写有误，正确应为“challenging”）
EMBO J. 1998 Nov 16;17(22):6427-36. doi: 10.1093/emboj/17.22.6427.