Carter J, McLaren A
J Reprod Fertil. 1975 Mar;42(3):439-45. doi: 10.1530/jrf.0.0420439.
The incorporation of tritiated thymidine into the uteri of immature mice in organ culture was assessed by autoradiography and liquid scintillation spectrometry. Oestrogenand progesterone, alone or combined, stimulated an increase in the incorporation of tritiated thymidine, indicating that DNA synthesis is occurring in vitro. Progesterone alone stimulated incorporation of label into stromal and epithelial cells, but the combination of progesterone and oestrogen stimulated the labelling of epithelial cells more than of stromal cells.
通过放射自显影术和液体闪烁光谱法评估了在器官培养中氚标记胸腺嘧啶核苷掺入未成熟小鼠子宫的情况。单独或联合使用雌激素和孕酮可刺激氚标记胸腺嘧啶核苷掺入量增加,表明体外发生了DNA合成。单独使用孕酮可刺激标记物掺入基质细胞和上皮细胞,但孕酮和雌激素联合使用时对上皮细胞的标记作用比对基质细胞的更强。