Sabri F, De Milito A, Pirskanen R, Elovaara I, Hagberg L, Cinque P, Price R, Chiodi F
Microbiology and Tumor Biology Center, Karolinska Institute, Nobels väg, 16, S 17177, Stockholm, Sweden.
J Neuroimmunol. 2001 Mar 1;114(1-2):197-206. doi: 10.1016/s0165-5728(00)00424-0.
We measured the levels of sFas and sFasL in CSF and serum of HIV-1 infected patients and related them to AIDS dementia complex (ADC). Specimens were obtained from 51 HIV-1 infected individuals (29 with ADC) and 39 HIV negative individuals. The sFas was detectable in all sera and 98% of CSF specimens. Measurable levels of sFasL were found in 79% of the CSF and 98% of sera samples. According to the presence or absence of ADC, we observed significant differences in CSF sFas (median and IQR 116, 132 vs. 30, 23 pg/ml, P<0.001) and sFasL (median and IQR 127, 290 vs. 15, 73 pg/ml, P<0.001) levels. The sFas in serum differed significantly between HIV-1 infected subjects and non-infected controls (P<0.001), with no correlation to ADC. On the contrary, sFasL in serum differed among HIV-1 infected subjects according to clinical signs of ADC. In the cross-sectional study, the number of cells present in CSF and CD4+ T cell counts in blood did not correlate to the levels of CSF sFas and sFasL. Interestingly, the number of HIV RNA copies in CSF correlated significantly to the levels of CSF sFasL (P=0.001) but not to sFas in the same compartment. Antiretroviral therapy reduced viral load and sFas levels in CSF in the majority of patients. sFas is a useful marker for ADC diagnosis and follow-up during antiviral treatment.
我们检测了HIV-1感染患者脑脊液和血清中的可溶性Fas（sFas）和可溶性Fas配体（sFasL）水平，并将其与艾滋病痴呆综合征（ADC）相关联。样本取自51名HIV-1感染个体（29例患有ADC）和39名HIV阴性个体。所有血清和98%的脑脊液样本中均可检测到sFas。在79%的脑脊液样本和98%的血清样本中发现了可测量水平的sFasL。根据是否存在ADC，我们观察到脑脊液中sFas（中位数和四分位间距分别为116、132与30、23 pg/ml，P<0.001）和sFasL（中位数和四分位间距分别为127、290与15、73 pg/ml，P<0.001）水平存在显著差异。HIV-1感染受试者与未感染对照组血清中的sFas存在显著差异（P<0.001），且与ADC无关。相反，血清中的sFasL在HIV-1感染受试者中根据ADC的临床症状而有所不同。在横断面研究中，脑脊液中的细胞数量和血液中的CD4+T细胞计数与脑脊液中sFas和sFasL的水平无关。有趣的是，脑脊液中HIV RNA拷贝数与脑脊液中sFasL水平显著相关（P=0.001），但与同一区域的sFas无关。抗逆转录病毒疗法在大多数患者中降低了病毒载量和脑脊液中的sFas水平。sFas是ADC诊断及抗病毒治疗期间随访的有用标志物。
