Benoliel R, Eliav E, Iadarola M J
Neuronal Gene Expression Unit, Pain and Neurosensory Mechanisms Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, MD, Bethesda, USA.
Pain. 2001 Mar;91(1-2):111-21. doi: 10.1016/s0304-3959(00)00417-6.
The aim of this study was to investigate neuropeptide Y (NPY) levels in trigeminal ganglia following infraorbital nerve injury. Two experimental procedures were performed in three groups of rats: a unilateral chronic constriction injury (CCI) to the infraorbital nerve (n=13), nerve manipulation without CCI (n=13) and unoperated controls (n=8). All rats underwent baseline and regular assessment of mechanical withdrawal threshold (Von Frey) and reaction to pin prick as well as free behavior evaluations. CCI to the infraorbital nerve induced significant hyperalgesia and allodynia within 9-12 days. At 6 days seven rats were euthanized and trigeminal ganglia harvested for immunocytochemical (ICC) studies. The study was ended at 14 days when all rats were euthanized and their ganglia harvested for ICC and radioimmunoassay (RIA) studies. An increase in NPY levels was seen in the ipsilateral ganglia of manipulated and CCI rats at 6 days, when rats displayed no pain-related behavior. At 14 days, ICC and RIA both detected significant increases in NPY levels in the ipsilateral ganglia of CCI and manipulated rats but not in unoperated controls. The possible roles of NPY in pain modulation and nerve injury are discussed in light of these findings.
本研究旨在调查眶下神经损伤后三叉神经节中神经肽Y（NPY）的水平。对三组大鼠进行了两种实验操作：对眶下神经进行单侧慢性压迫损伤（CCI，n = 13）、无CCI的神经操作（n = 13）以及未手术的对照组（n = 8）。所有大鼠均接受了机械性撤针阈值（von Frey）、针刺反应的基线和定期评估以及自由行为评估。眶下神经的CCI在9 - 12天内诱发了显著的痛觉过敏和异常性疼痛。在第6天，处死7只大鼠并采集三叉神经节用于免疫细胞化学（ICC）研究。在第14天，当所有大鼠被处死并采集其神经节用于ICC和放射免疫分析（RIA）研究时，研究结束。在第6天，当大鼠未表现出与疼痛相关的行为时，在接受操作和CCI的大鼠同侧神经节中观察到NPY水平升高。在第14天，ICC和RIA均检测到CCI和接受操作的大鼠同侧神经节中NPY水平显著升高，而未手术的对照组中未升高。根据这些发现，讨论了NPY在疼痛调节和神经损伤中的可能作用。
