Shirai K, Guerrero I, Huerta S, Saucedo G, Castillo A, Obdulia Gonzalez R, Hall G M.
Departamento de Biotecnología, Universidad Autonoma Metropolitana, D.F. Av. Michoacan y Purisima s/n Col., 09340, Vicentina, Mexico
Enzyme Microb Technol. 2001 Mar 8;28(4-5):446-452. doi: 10.1016/s0141-0229(00)00338-0.
Fermentation conditions and microorganisms were determined, based on acid production, glucose concentration as carbohydrate source. Inoculation levels to obtain a stable shrimp waste silage were also determined. Shrimp waste ensilation was an efficient method of preservation, allowing the recovery of chitin and another added-value products such as pigments, proteins and enzymes. From the various lactic acid bacteria tested, Lactobacillus pentosus and Lactobacillus sp. (B2) were the best lactic acid producers, although small quantities of acetic acid were detected in samples inoculated with Lactobacillus pentosus. Therefore B2 was chosen for the analysis of glucose consumption as well as for the determination of optimum inoculation levels. The best results were obtained at 10% (w/w wet basis) and 5% (v/w wet basis) respectively. Presence of starters and initial glucose concentration were critical factors in the fermentation of shrimp waste. High initial glucose and starter concentrations reduced the time and increased the amount of lactic acid produced. The fermentation pattern changed during ensilation from hetero to homofermentative. Shrimp waste ensilation prevented the growth of spoilage microorganisms keeping their microbial counts steady and pH values within the acid region.
根据产酸情况、作为碳水化合物来源的葡萄糖浓度确定了发酵条件和微生物。还确定了获得稳定虾废料青贮饲料的接种水平。虾废料青贮是一种有效的保存方法，可回收几丁质以及其他附加值产品，如色素、蛋白质和酶。在所测试的各种乳酸菌中，戊糖乳杆菌和乳杆菌属（B2）是最佳的乳酸产生菌，尽管在接种戊糖乳杆菌的样品中检测到少量乙酸。因此，选择B2进行葡萄糖消耗分析以及最佳接种水平的测定。分别在10%（湿重基础）和5%（体积/湿重基础）时获得了最佳结果。起始剂的存在和初始葡萄糖浓度是虾废料发酵的关键因素。高初始葡萄糖和起始剂浓度缩短了时间并增加了乳酸产量。青贮过程中发酵模式从异型发酵变为同型发酵。虾废料青贮可防止腐败微生物生长，使微生物数量保持稳定且pH值处于酸性区域。
