Lee S Y, Kim K K, Choe S J
Department of Oral Microbiology, College of Dentistry, Kangnung National University, Seoul, Republic of Korea.
Oral Microbiol Immunol. 2001 Apr;16(2):88-93. doi: 10.1034/j.1399-302x.2001.016002088.x.
The interaction of oral streptococci with human fibrinogen was investigated. Streptococcus gordonii was chosen as a representative species to study the binding to fibrinogen. S. gordonii DL1 adhered to immobilized fibrinogen and bovine serum albumin. Binding to immobilized fibrinogen was saturable, concentration and temperature dependent. The binding of S. gordonii DL1 to fibrinogen was inhibited by anti-fibrinogen antibody. Heating of the bacteria for 1 h at 95 degrees C resulted in 90% inhibition of the binding. Trypsin treatment of the bacteria resulted in decreased binding. Neither lipoteichoic acid nor culturing of the bacteria in a sucrose-supplemented medium had any effect on the binding. S. gordonii, Streptococcus sanguinis, Streptococcus mitis, and Streptococcus oralis bound to the immobilized fibrinogen, but mutans streptococci did not. None of the oral streptococci tested bound to the fibrinogen in fluid phase. These results suggest that the binding of S. gordonii DL1 to immobilized fibrinogen is mediated through a specific protein adhesin-receptor interaction, and fibrinogen acts as a cryptitope.
研究了口腔链球菌与人纤维蛋白原的相互作用。选择戈登链球菌作为代表性菌种来研究其与纤维蛋白原的结合。戈登链球菌DL1可黏附于固定化的纤维蛋白原和牛血清白蛋白。与固定化纤维蛋白原的结合具有饱和性，且依赖于浓度和温度。戈登链球菌DL1与纤维蛋白原的结合可被抗纤维蛋白原抗体抑制。将细菌在95℃加热1小时导致结合抑制90%。用胰蛋白酶处理细菌导致结合减少。脂磷壁酸以及在添加蔗糖的培养基中培养细菌对结合均无影响。戈登链球菌、血链球菌、缓症链球菌和口腔链球菌可与固定化纤维蛋白原结合，但变形链球菌不结合。所检测的口腔链球菌均不与液相中的纤维蛋白原结合。这些结果表明，戈登链球菌DL1与固定化纤维蛋白原的结合是通过特异性蛋白黏附素-受体相互作用介导的，且纤维蛋白原作为一个隐蔽表位。
