Hernández J. A., Talavera J. M., Martínez-Gómez P., Dicenta F., Sevilla F.
Departamento de Nutrición y Fisiología Vegetal, Centro de Edafología y Biología Aplicada del Segura, CSIC, Apartado 4195, E-30100 Murcia, Spain; Departamento de Mejora y Patología Vegetal, Centro de Edafología y Biología Aplicada del Segura, CSIC, Apartado 4195, E-30100 Murcia, Spain.
Physiol Plant. 2001 Mar;111(3):313-321. doi: 10.1034/j.1399-3054.2001.1110308.x.
Recent evidence has indicated that activated oxygen species (AOS) may function as molecular signals in the induction of defence genes. In the present work, the response of antioxidative enzymes to the plum pox virus (PPV) was examined in two apricot (Prunus armeniaca L.) cultivars, which behaved differently against PPV infection. In the inoculated resistant cultivar (Goldrich), a decrease in catalase (CAT) as well as an increase in total superoxide dismutase (SOD) and dehydroascorbate reductase (DHAR) activities were observed. Ascorbate peroxidase (APX), glutathione reductase (GR) and monodehydroascorbate reductase (MDHAR) did not change significantly in relation to non-inoculated (control) plants. In the susceptible cultivar (Real Fino), inoculation with PPV brought about a decrease in CAT, SOD and GR, whereas a rise in APX, MDHAR and DHAR activities was found in comparison to non-inoculated (control) plants. Apricot leaves contain only CuZn-SOD isozymes, which responded differently to PPV depending on the cultivar. Goldrich leaves contained 6 SODs and both SOD 1 and SOD 2 increased in the inoculated plants. In leaves from Real Fino, 5 SODs were detected and only SOD 5 was increased in inoculated plants. The different behaviour of SODs (H2O2-generating enzymes) and APX (an H2O2-remover enzyme) in both cultivars suggests an important role for H2O2 in the response to PPV of the resistant cultivar, in which no change in APX activity was observed. This result also points to further studies in order to determine if an alternative H2O2-scavenging mechanism takes place in the resistant apricot cultivar exposed to PPV. On the other hand, the ability of the inoculated resistant cultivar to induce SOD 1 and SOD 2 as well as the important increase of DHAR seems to suggest a relationship between these activities and resistance to PPV. This is the first report about the effect of PPV infection on the antioxidative enzymes of apricot plants. It opens the way for the further studies, which are necessary for a better understanding of the role of antioxidative processes in viral infection by PPV in apricot plants.
最近的证据表明，活性氧（AOS）可能作为诱导防御基因的分子信号。在本研究中，检测了两种对李痘病毒（PPV）表现不同的杏（Prunus armeniaca L.）品种中抗氧化酶对PPV的反应。在接种的抗性品种（Goldrich）中，过氧化氢酶（CAT）活性降低，而总超氧化物歧化酶（SOD）和脱氢抗坏血酸还原酶（DHAR）活性增加。抗坏血酸过氧化物酶（APX）、谷胱甘肽还原酶（GR）和单脱氢抗坏血酸还原酶（MDHAR）与未接种（对照）植株相比没有显著变化。在感病品种（Real Fino）中，接种PPV导致CAT、SOD和GR活性降低，而与未接种（对照）植株相比，APX、MDHAR和DHAR活性升高。杏叶仅含有CuZn-SOD同工酶，其对PPV的反应因品种而异。Goldrich叶片含有6种SOD，接种植株中SOD 1和SOD 2均增加。在Real Fino的叶片中，检测到5种SOD，接种植株中仅SOD 5增加。两个品种中SOD（产生H2O2的酶）和APX（清除H2O2的酶）的不同表现表明H2O2在抗性品种对PPV的反应中起重要作用，在该品种中未观察到APX活性变化。该结果还表明需要进一步研究，以确定在暴露于PPV的抗性杏品种中是否存在替代的H2O2清除机制。另一方面，接种的抗性品种诱导SOD 1和SOD 2的能力以及DHAR的显著增加似乎表明这些活性与对PPV的抗性之间存在关联。这是关于PPV感染对杏树抗氧化酶影响的首次报道。它为进一步研究开辟了道路，这对于更好地理解抗氧化过程在杏树中PPV病毒感染中的作用是必要的。
