Yew P R
Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX 78245-3207, USA.
J Cell Physiol. 2001 Apr;187(1):1-10. doi: 10.1002/1097-4652(2001)9999:9999<1::AID-JCP1049>3.0.CO;2-O.
Cell-cycle progression in all eukaryotes is driven by cyclin-dependent kinases (CDKs) and their cyclin partners. In vertebrates, the proper and timely duplication of the genome during S-phase relies on the coordinated activities of positive regulators such as CDK-cyclins and E2F, and negative regulators such as CDK inhibitors of the Cip/Kip and INK4 families. Recent and ongoing work indicates that many important regulators of G1- and S-phases are targeted for ubiquitination and subsequent degradation by the 26S proteasome. The proteolysis of key proteins during G1- and S-phases appears to be central for proper custodial regulation of DNA replication and the maintenance of cellular homeostasis in general. This review highlights the current literature regarding ubiquitin-mediated proteolysis of G1- and S-phase regulators and the control of events during the initiation and completion of DNA replication in vertebrates.
所有真核生物的细胞周期进程均由细胞周期蛋白依赖性激酶（CDK）及其细胞周期蛋白伴侣驱动。在脊椎动物中，S期基因组的正确及时复制依赖于诸如CDK-细胞周期蛋白和E2F等正调控因子以及诸如Cip/Kip和INK4家族的CDK抑制剂等负调控因子的协同作用。近期及正在进行的研究表明，许多G1期和S期的重要调控因子会被泛素化，并随后被26S蛋白酶体降解。在G1期和S期关键蛋白的蛋白水解作用对于DNA复制的正确监管调控以及维持细胞内稳态似乎至关重要。本综述重点介绍了有关泛素介导的G1期和S期调控因子蛋白水解作用以及脊椎动物DNA复制起始和完成过程中事件控制的当前文献。
