液相色谱/串联质谱法在生物分析定量中超快速梯度与快速等度色谱的比较

Ultra-fast gradient vs. fast isocratic chromatography in bioanalytical quantification by liquid chromatography/tandem mass spectrometry.

作者信息

Romanyshyn L, Tiller P R, Alvaro R, Pereira A, Hop C E

机构信息

Drug Metabolism, Merck Research Laboratories, RY80E-200, PO Box 2000, Rahway, NJ 07065-0900, USA.

出版信息

Rapid Commun Mass Spectrom. 2001;15(5):313-9. doi: 10.1002/rcm.229.

DOI:10.1002/rcm.229

PMID:11241760

Abstract

Liquid chromatography/tandem mass spectrometry (LC/MS/MS) methods developed for quantification using rapid ('ballistic') gradients on narrow bore, short HPLC columns have been previously described by this laboratory. This paper compares the fast gradient approach with the more traditional high-organic isocratic LC/MS/MS methods. The comparison is based on an analysis of the effectiveness of the chromatographic separations when using the two approaches (i.e. k', N, and W). The data presented herein are derived from actual biological samples analyzed as part of the drug discovery process.

摘要

本实验室之前已描述过利用窄内径短高效液相色谱（HPLC）柱上的快速（“弹道式”）梯度进行定量分析而开发的液相色谱/串联质谱（LC/MS/MS）方法。本文将这种快速梯度方法与更传统的高有机相等度LC/MS/MS方法进行了比较。该比较基于对使用这两种方法时色谱分离效果（即k'、N和W）的分析。本文所呈现的数据源自作为药物发现过程一部分所分析的实际生物样品。