Sirinarumitr T, Sorden S D, Morozov I, Paul P S
Department of Veterinary Pathology, Faculty of Veterinary Medicine, Kasetsart University, Kampangsaeng, Nakorn Pathom, Thailand.
J Vet Diagn Invest. 2001 Jan;13(1):68-71. doi: 10.1177/104063870101300113.
A double in situ hybridization method for the simultaneous detection of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus (PCV) genomes in the same tissue section was applied to lung tissues from 9 pigs in which PRRSV and PCV coinfection had been previously demonstrated. Paraffin-embedded tissue sections were simultaneously hybridized with a digoxigenin-labeled antisense RNA probe for PRRSV and a fluorescein-labeled antisense RNA probe for PCV, and hybridization was detected with anti-digoxigenin alkaline phosphatase/fast red and anti-fluorescein peroxidase/diaminobenzidine, respectively. PRRSV and PCV genomes were identified in the same pulmonary cell types as reported previously in all 9 pigs. In all pigs, PCV-positive cells outnumbered PRRSV-positive cells. A small proportion of alveolar macrophages contained both PRRSV and PCV genomes.
采用一种双重原位杂交方法，在同一组织切片中同时检测猪繁殖与呼吸综合征病毒（PRRSV）和猪圆环病毒（PCV）基因组，应用于9头先前已证实PRRSV和PCV共同感染的猪的肺组织。石蜡包埋的组织切片与地高辛标记的PRRSV反义RNA探针和荧光素标记的PCV反义RNA探针同时杂交，分别用抗地高辛碱性磷酸酶/固红和抗荧光素过氧化物酶/二氨基联苯胺检测杂交信号。在所有9头猪中，PRRSV和PCV基因组在与先前报道相同的肺细胞类型中被鉴定出来。在所有猪中，PCV阳性细胞数量多于PRRSV阳性细胞。一小部分肺泡巨噬细胞同时含有PRRSV和PCV基因组。
