Lyoo K S, Park Y H, Park B K
Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine, Seoul National University, Suwon 441-744, Korea.
J Vet Sci. 2001 Dec;2(3):201-7.
Porcine reproductive and respiratory syndrome virus(PRRSV)0, porcine circovirus type 2(PCV-2) and porcine parvovirus (PPV)0 infections were investigated as possible causes of the postweaning multisystemic wasting syndrome(PMWS). Specific primers for RT-PCR and PCR were designed for the differential detection of PRRSV, PCV-2 and PPV. Using PCR, these viruses were detected in homogenized tissue samples from pigs that had respiratory of reproductive problems in the time period between 1998 and 2000; the overall prevalences were: PRRSV 31.4%, PCV-2 46.5%, and PPV 8.1%. PCV-2 was also detected in aborted fetal tissues.
对猪繁殖与呼吸综合征病毒（PRRSV）、2型猪圆环病毒（PCV - 2）和猪细小病毒（PPV）感染作为断奶后多系统消耗综合征（PMWS）的可能病因进行了调查。设计了用于逆转录聚合酶链反应（RT - PCR）和聚合酶链反应（PCR）的特异性引物，以鉴别检测PRRSV、PCV - 2和PPV。利用PCR技术，在1998年至2000年期间出现呼吸或繁殖问题的猪的匀浆组织样本中检测到了这些病毒；总体患病率分别为：PRRSV 31.4%、PCV - 2 46.5%和PPV 8.1%。在流产的胎儿组织中也检测到了PCV - 2。
