豚鼠组胺N-甲基转移酶：cDNA克隆与mRNA分布

Guinea pig histamine N-methyltransferase: cDNA cloning and mRNA distribution.

作者信息

Kitanaka J, Kitanaka N, Tsujimura T, Kakihana M, Terada N, Takemura M

机构信息

Department of Pharmacology, Hyogo College of Medicine, Nishinomiya, Japan.

出版信息

Jpn J Pharmacol. 2001 Jan;85(1):105-8. doi: 10.1254/jjp.85.105.

DOI:10.1254/jjp.85.105

PMID:11243563

Abstract

We report here the isolation of histamine N-methyltransferase (HMT) cDNA from the guinea pig brain by the polymerase chain reaction on the basis of nucleotide sequences of rat and human counterparts. Guinea pig HMT consists of 292 amino acids, with homologies of 75.6% and 79.1% to rat and human HMT, respectively. Northern blotting analysis indicated that the 1.6-kb guinea pig HMT transcript was expressed at various levels in different tissues at the following relative abundance: jejunum, brain > lung, spleen, stomach > liver, kidney. HMT mRNA localized throughout the jejunum, and it was mainly expressed in epithelial cells and in Auerbach's plexus.

摘要

我们在此报告，基于大鼠和人类组胺N-甲基转移酶（HMT）的核苷酸序列，通过聚合酶链反应从豚鼠脑中分离出HMT cDNA。豚鼠HMT由292个氨基酸组成，与大鼠和人类HMT的同源性分别为75.6%和79.1%。Northern印迹分析表明，1.6kb的豚鼠HMT转录本在不同组织中以不同水平表达，其相对丰度如下：空肠、脑>肺、脾、胃>肝、肾。HMT mRNA定位于整个空肠，主要在上皮细胞和奥尔巴赫神经丛中表达。