[原位杂交法检测横纹肌肉瘤中MDM 2和p 53基因]
[Detection of MDM 2 and p 53 genes in rhabdomyosarcoma by in situ hybridization].
作者信息
Lai R, Wang Z, Zhe X
机构信息
Department of Pathology, Guangzhou General Hospital of PIA, Guangzhou 510010.
出版信息
Zhonghua Bing Li Xue Za Zhi. 1998 Apr;27(2):127-9.
OBJECTIVE
To observe the correlation between MDM2, p53 genes and the pathogenesis, clinical pathology and prognosis in rhabdomyosarcoma (RMS).
METHODS
Thirty one cases of MRS with follow-up history were covered for this study. MDM2 and p53 genes were detected by digoxigenin labeling in situ hybridization-technique.
RESULTS
The positive rates for MDM2 and p53 genes were 77.4% (24/31) and 66.7% (21/31) respectively, regardless of age, sex and the histological typing of RMS, but there was remarkable difference in the positive rates and intensity between the well and poorly differentiated cases, metastatic and non-metastatic cases as well as one year and three years survival period (P < 0.05).
CONCLUSIONS
The detection of MDM2 and p53 gene is considered valuable in evaluating the malignant degree, predictable metastasis and prognosis of RMS.
目的
观察MDM2、p53基因与横纹肌肉瘤（RMS）发病机制、临床病理及预后的相关性。
方法
本研究纳入31例有随访记录的横纹肌肉瘤患者。采用地高辛标记原位杂交技术检测MDM2和p53基因。
结果
无论患者年龄、性别及横纹肌肉瘤的组织学类型如何，MDM2和p53基因的阳性率分别为77.4%（24/31）和66.7%（21/31），但高分化与低分化病例、转移与非转移病例以及1年和3年生存期之间的阳性率及强度存在显著差异（P < 0.05）。
结论
检测MDM2和p53基因对评估横纹肌肉瘤的恶性程度、预测转移及预后具有重要价值。
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