[二甲基苯并蒽-十四烷酰佛波醇乙酯-亚硝基甲脲诱导的小鼠皮肤肿瘤中的p53基因突变]
[p53 gene mutations in mouse skin tumors induced by DMBA-TPA-MNNG].
作者信息
Zhang S, Klein-Szanto A
机构信息
Department of Pathology, West China University of Medical Sciences, Chengdu 610041.
出版信息
Zhonghua Bing Li Xue Za Zhi. 1998 Aug;27(4):298-300.
OBJECTIVE
To investigate the relationship between p53 gene mutations and mouse skin tumors induced by three-step carcinogenesis.
METHODS
The exons 5-8 of p53 gene were examined in 37 DMBA-TPA-MNNG induced mouse skin tumors [including 6 mice with papillomas, 15 mice having well differentiated squamous cell carcinomas (SCCI) and 16 mice with intermediately differentiated squamous cell carcinomas (SCC II)].
RESULTS
No p53 gene mutation was detected in the papilloma group, whereas 25.8% (8/31) of the SCC group had p53 gene mutations (4/15 of the SCC I mice and 4/16 of the SCC II mice). A total of 9 mutations were found in 8 mice with SCC, of which 7 were located in exon 8 and 7 were G-->A transitions.
CONCLUSION
The p53 gene mutations occurred during the process of malignant transformation from papilloma to SCC induced by three step carcinogenesis.
目的
研究p53基因突变与三步致癌法诱导的小鼠皮肤肿瘤之间的关系。
方法
检测37例经二甲基苯并蒽（DMBA）-十四酰佛波醇乙酯（TPA）-N-甲基-N'-硝基-N-亚硝基胍（MNNG）诱导产生的小鼠皮肤肿瘤（包括6例乳头状瘤小鼠、15例高分化鳞状细胞癌小鼠和16例中分化鳞状细胞癌小鼠）中p53基因的第5至8外显子。
结果
乳头状瘤组未检测到p53基因突变，而鳞状细胞癌组有25.8%（8/31）的小鼠发生p53基因突变（高分化鳞状细胞癌小鼠中4/15，中分化鳞状细胞癌小鼠中4/16）。在8例鳞状细胞癌小鼠中共发现9处突变，其中7处位于第8外显子，7处为G→A转换。
结论
在三步致癌法诱导的从乳头状瘤到鳞状细胞癌的恶性转化过程中发生了p53基因突变。
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