Chen Y, Zhou J, Yue B, Xiang Z, Song X, Li C, Wang B
Institute of Hematology, Xiehe Hospital, Tongji Medical University, Wuhan 430022, China.
Chin Med J (Engl). 1998 Aug;111(8):682-5.
To study the suppressing genes of apoptosis, namely Bcl-2, its family genes Bax, Bcl-Xl, and the inducing gene of apoptosis Fas/Apo-1.
The techniques of cytoimmuno-histiochemical stains, Western blotting and Northern blotting were used.
It was found that the antigens of Bcl-2 in acute myelogenous leukemia (AML) and acute lymphocytic (ALL) was higher than that in the normal (P < 0.01). At same time, Bcl-2 was obviously lower expression in complete remission (CR) group than that in non-remission (NR) one by retrospective analysis (P < 0.01). Though Bcl-2 was low expression in CR with Western blotting, there was no statistical significance (P > 0.05). In CR group the expression of Bcl-2 mRNA was obviously lower than that of NR one (P < 0.01). Even though leukemia expression of Bax with cytoimmuno-histiochemical stain was also lower than that in the normal people, there was no difference between CR and NR with cytoimmuno-histiochemical, Western blotting and Northern blotting (P > 0.05). There was difference of Bcl-Xl mRNA in two groups (P < 0.01). The expression Fas/Apo-1 in leukemia was lower than that in normal people (P < 0.01). But in CR and NR, there was no difference with cytoimmuno-histiochemical stain and Western blotting.
The Changes of genes and their proteins are significant theoretically and clinically. The antigen expression of Bcl-2 and the expression of Bcl-2 mRNA may be considered as a prognostic index for AML.
研究凋亡抑制基因，即Bcl-2、其家族基因Bax、Bcl-Xl以及凋亡诱导基因Fas/Apo-1。
采用细胞免疫组织化学染色、蛋白质印迹法和Northern印迹法技术。
发现急性髓系白血病（AML）和急性淋巴细胞白血病（ALL）中Bcl-2抗原高于正常水平（P<0.01）。同时，通过回顾性分析发现完全缓解（CR）组中Bcl-2的表达明显低于未缓解（NR）组（P<0.01）。尽管蛋白质印迹法显示CR组中Bcl-2表达较低，但无统计学意义（P>0.05）。CR组中Bcl-2 mRNA的表达明显低于NR组（P<0.01）。尽管细胞免疫组织化学染色显示白血病中Bax的表达也低于正常人，但细胞免疫组织化学、蛋白质印迹法和Northern印迹法显示CR组和NR组之间无差异（P>0.05）。两组之间Bcl-Xl mRNA存在差异（P<0.01）。白血病中Fas/Apo-1的表达低于正常人（P<0.01）。但在CR组和NR组中，细胞免疫组织化学染色和蛋白质印迹法无差异。
基因及其蛋白质变化在理论和临床上具有重要意义。Bcl-2的抗原表达和Bcl-2 mRNA表达可作为AML的预后指标。
