Lind Y, Engman J, Jorhem L, Glynn A W
Swedish National Food Administration, and Department of Environmental Toxicology, Uppsala University.
J Toxicol Environ Health A. 2001 Feb 23;62(4):269-80. doi: 10.1080/009841001459423.
The absorption and organ distribution of organic Cd from raw and broiled horse kidney was compared to that of CdCl2 at two dose levels (0.05 and 3 mg Cd/kg feed) in a feeding study in mice. The high Cd concentration in the horse kidney (raw 112 mg/kg; broiled 53 mg/kg) made it possible to mix kidney into mouse feed without marked effects on the composition of the feed. The weight of the mice, feed and water consumption, and Cd levels in the feed were determined once a week. After 9 wk of exposure, the liver and kidneys of the mice were sampled and Cd was analyzed. The Cd concentration in horse kidney was halved by broiling, and the content of soluble Cd decreased from 12% in raw kidney to 5% in broiled kidney. The majority of the soluble Cd was associated with proteins with the same molecular weight as metallothionein (MT) in both raw and broiled kidney. Broiling of the kidney had no marked effect on the fractional accumulation of organic Cd in mice. The fractional accumulation of inorganic CdCl2, on the other hand, was significantly higher than that of organic Cd in the low dose groups but not in the high dose groups. The ratio between Cd accumulation in kidney and that in liver was higher in the group receiving raw kidney compared to the ratio in the group receiving CdCl2 at both the high and low exposure levels. This indicates that the raw kidney contained a Cd form that was more preferentially distributed to the kidneys.
在一项针对小鼠的喂养研究中，比较了生马肾和烤马肾中有机镉的吸收及器官分布情况，以及在两种剂量水平（0.05和3毫克镉/千克饲料）下氯化镉的吸收及器官分布情况。马肾中镉的高浓度（生马肾为112毫克/千克；烤马肾为53毫克/千克）使得将马肾混入小鼠饲料中而不对饲料成分产生显著影响成为可能。每周测定一次小鼠的体重、饲料和水的消耗量以及饲料中的镉含量。暴露9周后，对小鼠的肝脏和肾脏进行采样并分析镉含量。烤马肾后，马肾中的镉浓度减半，可溶性镉含量从生马肾中的12%降至烤马肾中的5%。在生马肾和烤马肾中，大部分可溶性镉都与分子量与金属硫蛋白（MT）相同的蛋白质结合。烤马肾对小鼠体内有机镉的累积分数没有显著影响。另一方面，在低剂量组中，无机氯化镉的累积分数显著高于有机镉，但在高剂量组中并非如此。在高暴露水平和低暴露水平下，接受生马肾的组中肾脏与肝脏镉累积量的比值均高于接受氯化镉的组。这表明生马肾中含有一种更优先分布于肾脏的镉形态。
