Oettmeier W, Masson K, Hecht H
Lehstuhl Biochemie der Pflanzen, Ruhruniversität, Bochum, Germany.
Biochim Biophys Acta. 2001 Apr 2;1504(2-3):346-51. doi: 10.1016/s0005-2728(00)00263-2.
Members of the new chemical class of 7-substituted 6-bromo-benzo[4,5]imidazo[1,2alpha]pyridin-8,9-diones were found to be excellent inhibitors at the Q(B) site of the photosystem II D1 reaction center protein. The best inhibitors with pI(50)-values of >7 are: dimethyl-propyl, 7.05; i-pentyl, 7.36; t. butyl, 7.47; and i-propyl, 7.51. Displacement experiments with [14C]atrazine revealed that the 8,9-diones behave non-competitively in respect of Photosystem II herbicides and, hence, have to be considered as a new type of Photosystem II inhibitors. This notion is further corroborated by their inhibitory activity in D1 mutants of Chlamydomonas reinhardtii.
人们发现，7-取代的6-溴苯并[4,5]咪唑并[1,2α]吡啶-8,9-二酮这一新化学类别的成员是光系统II D1反应中心蛋白Q(B)位点的优秀抑制剂。pI(50)值>7的最佳抑制剂有：二甲基丙基，7.05；异戊基，7.36；叔丁基，7.47；以及异丙基，7.51。用[14C]莠去津进行的置换实验表明，8,9-二酮对光系统II除草剂表现出非竞争性，因此，必须将其视为一种新型的光系统II抑制剂。莱茵衣藻D1突变体中的抑制活性进一步证实了这一观点。
