在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳之前，用连苯三酚红沉淀法测定脑脊液中的蛋白质浓度。
Protein concentration of cerebrospinal fluid by precipitation with Pyrogallol Red prior to sodium dodecyl sulphate-polyacrylamide gel electrophoresis.
作者信息
Williams K M, Marshall T
机构信息
Analytical Biochemistry Group, Institute of Pharmacy, Chemistry and Biomedical Sciences, School of Sciences, The University of Sunderland, SR1 3RG, Sunderland, UK.
出版信息
J Biochem Biophys Methods. 2001 Feb 26;47(3):197-207. doi: 10.1016/s0165-022x(00)00135-4.
The Pyrogallol Red Molybdate (PRM) and Coomassie Brilliant Blue (CBB) protein dye-binding assays have been applied to samples of cerebrospinal fluid (CSF) to investigate protein concentration by dye precipitation prior to sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The protein concentration values of the CSF samples (N=62) showed good agreement between the PRM and CBB assays as indicated by linear regression analysis (y(PRM)=1.033x(CBB)+1.004 in units of mg/l, r=0.99) but the PRM assay was optimal for protein concentration as the PRM protein-dye complex was less soluble allowing protein recovery over a wider working range. Dye precipitation using PRM is recommended as a simple, rapid and economic method for protein concentration of samples of CSF prior to SDS-PAGE.
焦棓酚红钼酸盐（PRM）和考马斯亮蓝（CBB）蛋白质染料结合测定法已应用于脑脊液（CSF）样本，以便在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）之前通过染料沉淀来研究蛋白质浓度。脑脊液样本（N = 62）的蛋白质浓度值在PRM和CBB测定之间显示出良好的一致性，线性回归分析表明（以mg/l为单位，y(PRM)=1.033x(CBB)+1.004，r = 0.99），但PRM测定对于蛋白质浓度而言是最佳的，因为PRM蛋白质-染料复合物的溶解度较低，从而允许在更宽的工作范围内回收蛋白质。建议使用PRM进行染料沉淀，作为在SDS-PAGE之前对脑脊液样本进行蛋白质浓缩的一种简单、快速且经济的方法。
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