Qu X, Zhang C, Zhai Y, Xing G, Wei H, Yu Y, Wu S, He F
Department of Genomics and Proteomics, Beijing Institute of Radiation Medicine, Chinese National Human Genome Center at Beijing, 27 Taiping Road, 100850, P.R., Beijing, China.
Gene. 2001 Feb 7;264(1):37-44. doi: 10.1016/s0378-1119(01)00324-9.
We report the molecular characterization of a novel human homologue of mouse npdc1 (neural proliferation, differentiation and control, 1) gene, designated human npdc1 (hnpdc1). hnpdc1 was identified by large-scale sequencing of fetal liver cDNA libraries and the full-length cDNA was obtained by PCR amplification. The hnpdc1 gene, which contains nine exons, was mapped to human chromosome 15. It encodes a polypeptide of 325 amino acids, which shows high homology (77% identity) to the mouse NPDC1. Sequence analysis has shown that hNPDC1 protein contains a putative signal peptide of 34 amino acids, a transmembrane segment, and a typical bipartite nuclear localization signal. Northern blot and dot blot hybridization indicates that, just like mnpdc1, hnpdc1 mRNA is strongly expressed in adult brain (especially in hippocampus, frontal lobe and temporal lobe) and about 1.82-fold higher in adult brain than that in fetal brain. Unlike mnpdc1, however, hnpdc1 contains two transcripts instead of only one (1.5 kb), and has high expression levels in prostate, pituitary gland, and mammary glands. These results support that hNPDC1 plays a role in the control of neural cell proliferation and differentiation, and suggest that it may be involved in the development of several secretion glands.
我们报道了一种新的人类小鼠npdc1（神经增殖、分化与控制，1）基因同源物的分子特征，命名为人类npdc1（hnpdc1）。hnpdc1是通过对胎儿肝脏cDNA文库进行大规模测序鉴定出来的，全长cDNA通过PCR扩增获得。hnpdc1基因包含9个外显子，定位于人类染色体15。它编码一个由325个氨基酸组成的多肽，与小鼠NPDC1具有高度同源性（77%一致性）。序列分析表明，hNPDC1蛋白包含一个由34个氨基酸组成的假定信号肽、一个跨膜区段和一个典型的双分型核定位信号。Northern印迹和斑点印迹杂交表明，与mnpdc1一样，hnpdc1 mRNA在成体脑中强烈表达（尤其是在海马体、额叶和颞叶），在成体脑中的表达水平比胎儿脑高约1.82倍。然而，与mnpdc1不同的是，hnpdc1包含两个转录本而非仅一个（1.5 kb），并且在前列腺、垂体和乳腺中具有高表达水平。这些结果支持hNPDC1在神经细胞增殖和分化的控制中发挥作用，并表明它可能参与了多个分泌腺的发育。
