Nates S F, McKenney C L
US Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, FL 32561-5299, USA.
Comp Biochem Physiol C Toxicol Pharmacol. 2000 Dec;127(3):317-25. doi: 10.1016/s0742-8413(00)00157-2.
This study examines the effects of Fenoxycarb on larval growth, and lipid class and fatty acid composition in first crabs of the mud crab Rhithropanopeus harrisii reared through total larval development in nominal water concentrations from 1 to 100 microg/l. In first crabs of R. harrisii, dry weight (microg) decreased significantly (P < 0.05) from 228.8+/-38.2 microg (n = 9) in the controls to 131.8+/-10.1 microg (n = 4) in animals exposed throughout larval development to 100 microg/l. A significant (P < 0.05) reduction was found between total lipid content in the controls and first crabs reared at concentrations greater than 50 microg/l. In relative terms (% dry weight), different lipid classes predominated in the controls and the various fenoxycarb exposure concentrations. There were no significant (P > 0.05) differences among the treatment groups in phospholipid level, while the triglyceride content was significantly lower in crabs exposed to 10 and 100 microg/l. No significant differences in the percent of free fatty acids were found in crabs exposed to 1-10 microg/l and the controls. Free sterols in crabs exposed to concentrations higher than 10 microg/l were below the detection limit. Control animal fatty acid profiles were dominated by palmitic, stearic, and oleic acid, accounting for 48% of total fatty acids (TFA). The fatty acid composition of crabs exposed to 100 microg/l significantly (P < 0.05) differed from the controls. The results suggest that fenoxycarb has substantial effects on growth, lipid class and fatty acid composition in developing larvae of R. harrisii at water concentrations greater than 10 microg/l.
本研究考察了烯虫酯对哈氏方蟹（Rhithropanopeus harrisii）幼体生长以及经整个幼体发育阶段饲养在名义水体浓度为1至100微克/升环境中的初孵蟹类脂质类别和脂肪酸组成的影响。在哈氏方蟹的初孵蟹中，干重（微克）显著下降（P < 0.05），从对照组的228.8±38.2微克（n = 9）降至在整个幼体发育阶段暴露于100微克/升环境中的动物的131.8±10.1微克（n = 4）。在对照组和饲养于浓度大于50微克/升环境中的初孵蟹之间，总脂质含量存在显著（P < 0.05）降低。相对而言（干重百分比），对照组和不同烯虫酯暴露浓度下，不同脂质类别占主导地位。各处理组间磷脂水平无显著（P > 0.05）差异，而暴露于10和100微克/升的蟹类中甘油三酯含量显著较低。暴露于1至10微克/升的蟹类和对照组中，游离脂肪酸百分比无显著差异。暴露于浓度高于10微克/升的蟹类中游离甾醇低于检测限。对照动物的脂肪酸谱以棕榈酸、硬脂酸和油酸为主，占总脂肪酸（TFA）的48%。暴露于100微克/升的蟹类的脂肪酸组成与对照组显著（P < 0.05）不同。结果表明，在水体浓度大于10微克/升时，烯虫酯对哈氏方蟹发育幼体的生长、脂质类别和脂肪酸组成具有显著影响。
