Blokhuis T J, den Boer F C, Bramer J A, Jenner J M, Bakker F C, Patka P, Haarman H J
Department of Surgery/Traumatology, Academic Hospital Vrije Universiteit, Amsterdam, The Netherlands.
Biomaterials. 2001 Apr;22(7):725-30. doi: 10.1016/s0142-9612(00)00238-6.
Fracture healing could be stimulated with osteoinductive bone morphogenetic proteins (bmp's), such as osteogenic protein-1 (OP-1), but little is known about its effectiveness in stimulation of fracture healing. In this study, biomechanical and histological aspects of fracture healing after an injection of OP-1 in the fracture gap were investigated. In 40 goats, a closed fracture was created in the left tibia. The fractures were stabilized with an external fixator and the animals were assigned to four different groups: no injection, injection of 1 mg OP-1, injection of 1 mg OP-1 with collagenous carrier material, and injection of carrier material alone. Twenty-one animals were sacrificed after 2 weeks and 19 after 4 weeks. Biomechanical testing was perfomed on both explanted tibiae. Four longitudinal samples of the fracture were sawn, processed for histology, and examined by two observers. Biomechanical evaluation showed a higher stiffness and strength at 2 weeks after injection of OP-1. Histological evaluation showed normal fracture healing patterns in all animals without adverse effects of the given injections. These data show that fracture healing can be accelerated with a single injection of OP-1, eventually resulting in normally healed bone.
骨诱导性骨形态发生蛋白（BMPs），如成骨蛋白-1（OP-1），可以刺激骨折愈合，但关于其刺激骨折愈合的有效性知之甚少。在本研究中，研究了在骨折间隙注射OP-1后骨折愈合的生物力学和组织学方面。在40只山羊的左胫骨制造闭合性骨折。用外固定器固定骨折，将动物分为四组：不注射、注射1mg OP-1、注射1mg OP-1与胶原载体材料、仅注射载体材料。21只动物在2周后处死，19只在4周后处死。对两根取出的胫骨都进行了生物力学测试。锯取骨折处的四个纵向样本，进行组织学处理，并由两名观察者检查。生物力学评估显示，注射OP-1后2周时刚度和强度更高。组织学评估显示所有动物骨折愈合模式正常，所给注射无不良反应。这些数据表明，单次注射OP-1可以加速骨折愈合，最终使骨折正常愈合。
