低亲和力神经生长因子受体（p75NGFR）对沙土鼠海马缺血后迟发性神经元死亡的作用。

The contribution of low affinity NGF receptor (p75NGFR) to delayed neuronal death after ischemia in the gerbil hippocampus.

作者信息

Bagum M A, Miyamoto O, Toyoshima T, Masada T, Nagahata S, Itano T

机构信息

Department of Oral Surgery, Kagawa Medical University, Japan.

出版信息

Acta Med Okayama. 2001 Feb;55(1):19-24. doi: 10.18926/AMO/32030.

DOI:10.18926/AMO/32030

PMID:11246973

Abstract

The implication of low affinity nerve growth factor receptor (p75NGFR), which is believed to play a pro-apoptotic role, in delayed neuronal death (DND) after ischemia in the gerbil hippocampus was investigated. Immunohistochemistry and Western blot analysis revealed that the presence of p75 NGFR immunoreactivity (IR) was negligible in the hippocampus of the sham control gerbil but appeared clearly in CA1 neurons 3 and 4 days after 5-min transient ischemia. Terminal deoxynucleotidyl transferase-mediated UTP nick end labeling (TUNEL) positive nuclei appeared when the level of p75NGFR IR increased. Furthermore, almost all TUNEL-positive CA1 neurons also costained for p75NGFR. These results suggest that p75NGFR contributes to DND after ischemia by an apoptotic mechanism.

摘要

低亲和力神经生长因子受体（p75NGFR）被认为具有促凋亡作用，本研究探讨了其在沙土鼠海马缺血后迟发性神经元死亡（DND）中的影响。免疫组织化学和蛋白质印迹分析显示，在假手术对照沙土鼠的海马中，p75 NGFR免疫反应性（IR）微乎其微，但在5分钟短暂缺血后3天和4天，CA1神经元中p75 NGFR免疫反应性明显出现。当p75NGFR IR水平升高时，末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）阳性细胞核出现。此外，几乎所有TUNEL阳性的CA1神经元也对p75NGFR进行了共染色。这些结果表明，p75NGFR通过凋亡机制促成缺血后的DND。