Rafałowska J, Fidziańska A, Dziewulska D, Podlecka A, Jamrozik Z
Department of Neuropathology and Neuromuscular Units, Medical Research Centre, Polish Academy of Sciences, Warszawa.
Pol J Pathol. 2000;51(3):145-51.
We investigated the development of vascular basement membrane in immature spinal cord vessels during rat spinal cord myelination. Correlative ultrastructural and immunohistochemical study indicated that fibronectin was the first component of extracellular matrix. Then, on the 9th postnatal day, laminin appeared. At that time, lamina lucida of vascular basement membrane was not detectable. On the 15th day, when collagen IV was visible, lamina densa and lamina lucida were occasionally observed. All components of basement membrane--fibronectin, laminin, collagen IV, alpha-2 laminin (merosin)--and ultrastructural division into two layers were detected on the 25th postnatal day. The results of this study indicates that a gradual development of endothelium in immature rat spinal cord blood vessels leads to a gradual increase of synthesis of extracellular matrix components.
我们研究了大鼠脊髓髓鞘形成过程中未成熟脊髓血管中血管基底膜的发育情况。相关的超微结构和免疫组织化学研究表明，纤连蛋白是细胞外基质的首个成分。然后，在出生后第9天，层粘连蛋白出现。此时，血管基底膜的透明层不可见。在第15天，当可见IV型胶原时，偶尔可观察到致密层和透明层。在出生后第25天，检测到基底膜的所有成分——纤连蛋白、层粘连蛋白、IV型胶原、α-2层粘连蛋白（merosin）——以及超微结构上分为两层的情况。本研究结果表明，未成熟大鼠脊髓血管内皮的逐渐发育导致细胞外基质成分合成的逐渐增加。
