Vehmeyer S B, Bloem R M, Petit P L
The Netherlands Bone Bank Foundation, The Netherlands.
J Hosp Infect. 2001 Mar;47(3):193-7. doi: 10.1053/jhin.2000.0836.
This study assesses the value of blood cultures in combination with swab culturing techniques in association with bone banking procedures. The results of blood and swab cultures of two postmortem bone donors were compared with procured grafts, cultured in their entirety. In one donor, who died of drowning, three of the 12 entire graft cultures were positive with the same microorganism of high pathogenicity as the blood culture, whilst the swab culture of only one graft was positive. In the second donor, who died from myocardial infarction, four entire cultures were positive with the same organism of high pathogenicity as the blood culture, whilst the swab cultures of three grafts were positive. In both donors identical organisms were cultured from the myocardium of the pulmonary or aortic heart valve. The results confirm the limited sensitivity of swab culturing techniques. Especially micro-organisms inside a graft, disseminated through the bloodstream, can remain unnoticed. Blood cultures seem to provide additional information on micro-organisms that have spread haematogenously pre-mortem and may even provide information on the source from which they have spread.
本研究评估了血培养结合拭子培养技术在骨库程序中的价值。将两名尸体骨供体的血培养和拭子培养结果与完整培养的获取移植物进行了比较。在一名死于溺水的供体中，12个完整移植物培养物中有3个与血培养中相同的高致病性微生物呈阳性，而只有一个移植物的拭子培养呈阳性。在第二名死于心肌梗死的供体中，4个完整培养物与血培养中相同的高致病性微生物呈阳性，而3个移植物的拭子培养呈阳性。在两名供体中，均从肺或主动脉心脏瓣膜的心肌中培养出相同的微生物。结果证实了拭子培养技术的敏感性有限。特别是移植物内通过血流传播的微生物可能未被发现。血培养似乎能提供有关生前血行播散微生物的额外信息，甚至可能提供它们的传播来源信息。
