Schlag M G, Harris K A, Potter R F
Department of Medical Biophysics, University of Western Ontario, London, Ontario N6A 4G5, Canada.
Am J Physiol Heart Circ Physiol. 2001 Apr;280(4):H1716-21. doi: 10.1152/ajpheart.2001.280.4.H1716.
The role of leukocytes and nonleukocyte-derived reactive oxygen metabolites (ROMs) in reperfusion-induced skeletal muscle injury was determined. Male rats received 2 h no-flow hindlimb ischemia-reperfusion (I/R, n = 6) or were rendered neutropenic via antineutrophil serum (ANS) before I/R (I/R + ANS, n = 5). Oxygen radicals in the absence of neutrophils were tested by administration of dimethylthiourea (DMTU) (I/R + ANS + DMTU, n = 5). Perfused capillaries (CD(per)) and rolling (L(r)), adherent (L(a)), and extravasated leukocytes (L(e)) in the extensor digitorum longus muscle were measured every 15 min during 90 min of reperfusion using intravital microscopy. The vital dyes bisbenzimide (BB) and ethidium bromide (EB) provided direct measures of tissue injury (EB/BB). CD(per) decreased immediately on reperfusion in the I/R and I/R + ANS groups. CD(per) in the I/R + ANS + DMTU group remained at baseline throughout reperfusion. L(a) increased in the I/R group; however, EB/BB was the same between I/R and I/R + ANS groups. Injury in the I/R + ANS + DMTU group did not differ from other groups > or =60 min, after which EB/BB became significantly lower. L(e) did not differ between groups and was highly correlated to tissue injury. The results suggest that L(e) lead to parenchymal injury, and ROMs lead to perfusion deficits during the early reperfusion period after ischemia.
研究了白细胞和非白细胞来源的活性氧代谢产物（ROMs）在再灌注诱导的骨骼肌损伤中的作用。雄性大鼠接受2小时无血流的后肢缺血再灌注（I/R，n = 6），或在I/R前通过抗中性粒细胞血清（ANS）使其中性粒细胞减少（I/R + ANS，n = 5）。通过给予二甲基硫脲（DMTU）来检测无中性粒细胞时的氧自由基（I/R + ANS + DMTU，n = 5）。在再灌注的90分钟内，每隔15分钟使用活体显微镜测量趾长伸肌中的灌注毛细血管（CD(per)）以及滚动（L(r)）、黏附（L(a)）和渗出的白细胞（L(e)）。活性染料双苯甲酰胺（BB）和溴化乙锭（EB）提供了组织损伤的直接测量指标（EB/BB）。I/R组和I/R + ANS组在再灌注时CD(per)立即下降。I/R + ANS + DMTU组的CD(per)在整个再灌注过程中保持在基线水平。I/R组的L(a)增加；然而，I/R组和I/R + ANS组之间的EB/BB相同。I/R + ANS + DMTU组在60分钟及以后的损伤与其他组无差异，此后EB/BB显著降低。各组之间的L(e)无差异，且与组织损伤高度相关。结果表明，L(e)导致实质损伤，而ROMs在缺血后的早期再灌注期导致灌注不足。
