Ono T, Kurashige T, Harada N, Noguchi Y, Saika T, Niikawa N, Aoe M, Nakamura S, Higashi T, Hiraki A, Wada H, Kumon H, Old L J, Nakayama E
Department of Immunology, Okayama University Medical School, Okayama 700-8558, Japan.
Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3282-7. doi: 10.1073/pnas.041625098.
Serological expression cloning of antigens eliciting a humoral immune response to a syngeneic mouse sarcoma identified pem (mouse placenta and embryonic expression gene) as a new member of the cancer/testis family. To identify the human homologue of pem, mouse pem sequences and pem-related expressed sequence tags from human testis were used as PCR primers for amplification using human testis cDNA. However, rather than pem, another gene, designated OY-TES-1, was isolated and found to be the human homologue of proacrosin binding protein sp32 precursor originally identified in mouse, guinea pig, and pig. OY-TES-1 maps to chromosome 12p12-p13 and contains 10 exons. Southern blot analysis suggests the presence of two OY-TES-1-related genes in the human genome. In normal tissues, OY-TES-1 mRNA was expressed only in testis, whereas in malignant tissues, a variable proportion of a wide array of cancers, including bladder, breast, lung, liver, and colon cancers, expressed OY-TES-1. Serological survey of 362 cancer patients with a range of different cancers showed antibody to OY-TES-1 in 25 patients. No OY-TES-1 sera reactivity was found in 20 normal individuals. These findings indicate that OY-TES-1 is an additional member of the cancer/testis family of antigens and that OY-TES-1 is immunogenic in humans.
通过血清学表达克隆技术，鉴定出一种能引发对同基因小鼠肉瘤产生体液免疫反应的抗原，该抗原确定Pem（小鼠胎盘和胚胎表达基因）为癌症/睾丸抗原家族的新成员。为了鉴定Pem的人类同源物，使用小鼠Pem序列和来自人类睾丸的Pem相关表达序列标签作为PCR引物，以人类睾丸cDNA进行扩增。然而，分离得到的不是Pem，而是另一个名为OY-TES-1的基因，它被发现是最初在小鼠、豚鼠和猪中鉴定出的前顶体蛋白结合蛋白sp32前体的人类同源物。OY-TES-1定位于染色体12p12 - p13，包含10个外显子。Southern印迹分析表明人类基因组中存在两个与OY-TES-1相关的基因。在正常组织中，OY-TES-1 mRNA仅在睾丸中表达，而在恶性组织中，包括膀胱癌、乳腺癌、肺癌、肝癌和结肠癌在内的多种癌症中有不同比例表达OY-TES-1。对362例患有不同癌症的癌症患者进行的血清学调查显示，25例患者体内存在抗OY-TES-1抗体。在20名正常个体中未发现OY-TES-1血清反应性。这些发现表明OY-TES-1是癌症/睾丸抗原家族的另一个成员，并且OY-TES-1在人类中具有免疫原性。
