Daali Y, Cherkaoui S, Veuthey J L
Laboratory of Pharmaceutical Analytical Chemistry, University of Geneva, Bd d'Yvoy 20, 1211 Geneva 4, Switzerland.
J Pharm Biomed Anal. 2001 Mar;24(5-6):849-56. doi: 10.1016/s0731-7085(00)00552-5.
Capillary zone electrophoresis was applied to separate caseinoglycomacropeptide glycoforms and characterize microheterogeneity of the glycopeptide. Particular attention was paid to the sialic acid content in caseinoglycomacropeptide obtained through different manufacturing processes. A chemometric approach was used to simultaneously study effects of acid concentration, hydrolysis time and temperature on sialic acid release from caseinoglycomacropeptide. Hydrolysis conditions that maximize sialic acid release were chosen. Sialic acid was determined using high performance anion exchange chromatography coupled with pulsed amperometric detection. Results were compared to those obtained by alternative techniques, such as colorimetric and enzymatic methods.
采用毛细管区带电泳分离酪蛋白糖巨肽糖型并表征糖肽的微观异质性。特别关注了通过不同生产工艺获得的酪蛋白糖巨肽中的唾液酸含量。采用化学计量学方法同时研究酸浓度、水解时间和温度对酪蛋白糖巨肽中唾液酸释放的影响。选择了能使唾液酸释放最大化的水解条件。使用高效阴离子交换色谱结合脉冲安培检测法测定唾液酸。将结果与通过比色法和酶法等替代技术获得的结果进行比较。
