Truant A L, Conaron J, Moghaddas J
Clinical Microbiology and Immunology Laboratories, Temple University Hospital, Philadelphia, PA 19140, USA.
Diagn Microbiol Infect Dis. 2001 Feb;39(2):121-3. doi: 10.1016/s0732-8893(00)00230-3.
Five species of mycobacteria were stained by Ziehl-Neelsen, Kinyoun and Auramine-Rhodamine after either routine autoclaving, or exposure to rifabutin and ciprofloxacin. Organisms from each treatment group were stained and compared to viable controls. ATCC strains and fresh clinical isolates of all five species were evaluated. All species in both treatment protocols presented similar staining characteristics. Viability of mycobacteria, therefore, must be established by culture.
五种分枝杆菌在常规高压灭菌后，或暴露于利福布汀和环丙沙星后，采用萋尼氏染色法、金胺 - 罗丹明染色法和金胺 - 酚染色法进行染色。对每个治疗组的菌株进行染色，并与活的对照进行比较。评估了所有五个菌种的美国典型培养物保藏中心（ATCC）菌株和新鲜临床分离株。两种治疗方案中的所有菌种均呈现相似的染色特征。因此，分枝杆菌的活力必须通过培养来确定。
