Selznick S H, Thatcher M L, Brown K S, Haussler C A
Selznick Scientifc Software, LLC, Tucson, Arizona 85719, USA.
In Vitro Cell Dev Biol Anim. 2001 Jan;37(1):55-61. doi: 10.1290/1071-2690(2001)037<0055:DAAOCS>2.0.CO;2.
Prototype computer software for a Cell Culture Laboratory Management System (CCLMS) has been developed to relieve cell culture specialists of the burden of manual recordkeeping. Conventional data archives in cell culture laboratories are prone to error and expensive to maintain. The reliance upon cell culture to provide models for biochemical and molecular biological research serves to magnify errors at great expense. The CCLMS prototype encapsulates a modular software application that manages the many aspects of cell culture laboratory recordkeeping. A transaction-based database stores detailed information on subcultures, freezes and thaws, prints waterproof labels for culture vessels, and provides for immediate historical trace-back of any cultured cell line. Linked database files store information specific to an individual culture flask while removing redundancy between similar groups of flasks. A frozen cell log maintains locations of all vials within any type of cryogenic storage unit, locates spaces for newly frozen cell lines, and generates alphabetical or numerical reports. Finally, modules for maintaining cell counts, user records, and culture vessel specifications to support a comprehensive automation process are incorporated within this software. The developed CCLMS prototype has been demonstrated to be an adaptable, reliable tool for improving training, efficiency, and historical rigor for two independent cell culture facilities.
已开发出用于细胞培养实验室管理系统（CCLMS）的原型计算机软件，以减轻细胞培养专家手工记录的负担。细胞培养实验室中的传统数据存档容易出错且维护成本高昂。依赖细胞培养来为生化和分子生物学研究提供模型会导致错误大量增加，成本也很高。CCLMS原型封装了一个模块化软件应用程序，用于管理细胞培养实验室记录的诸多方面。一个基于事务的数据库存储有关传代培养、冻存和解冻的详细信息，为培养容器打印防水标签，并提供对任何培养细胞系的即时历史追溯。链接的数据库文件存储特定于单个培养瓶的信息，同时消除相似培养瓶组之间的冗余。一个冻存细胞日志记录任何类型低温存储单元内所有小瓶的位置，为新冻存的细胞系找到存放空间，并生成按字母顺序或数字顺序排列的报告。最后，该软件还包含用于维护细胞计数、用户记录和培养容器规格以支持全面自动化过程的模块。已证明所开发的CCLMS原型是一种适应性强、可靠的工具，可用于提高两个独立细胞培养设施的培训水平、效率和历史严谨性。
