Baum C, Richters A, Ostertag W
Heinrich-Pette-Institute, Department Cell & Virus Genetics, Martinistrasse 52, D-20251 Hamburg, Germany.
Curr Opin Mol Ther. 1999 Oct;1(5):605-12.
Gene transfer vectors based on simple retroviruses and more complex lentiviruses are currently the most reliable tools for stable establishment of transgenes in hematopoietic cells. While important hurdles in basic gene transfer technologies have been overcome in recent years, there is still some uncertainty in the choice of the cis-regulatory elements of the vector. These elements dictate the overall level, clonal variability, response to differentiation and persistence of transgene expression in vivo and thus have a significant influence on the outcome of therapeutic applications of somatic gene transfer. The rationale underlying the further improvement of such cis-elements is reviewed here.
基于简单逆转录病毒和更复杂慢病毒的基因转移载体，目前是在造血细胞中稳定建立转基因最可靠的工具。尽管近年来基础基因转移技术中的重要障碍已被克服，但载体顺式调控元件的选择仍存在一些不确定性。这些元件决定了转基因在体内表达的总体水平、克隆变异性、对分化的反应以及持久性，因此对体细胞基因转移治疗应用的结果有重大影响。本文综述了进一步改进此类顺式元件的基本原理。
