Singh S K, Chakravarty S
Department of Zoology, Banaras Hindu University, Varanasi, India.
Asian J Androl. 2001 Mar;3(1):39-44.
To study the effect of 5-nitro-2-furaldehyde semicarbazone (nitrofurazone), a derivative of nitrofuran, on male reproductive organs of Parkes (P) strain mice.
Mice were given nitrofurazone orally at a dose of 64 mg/kg body weight per day, for 10 and 20 days, and were killed 24 h and/or 56 days after the last treatment. Histological appearance of testis, motility and number of spermatozoa in cauda epididymidis, and biochemical indices in epididymis and seminal vesicle were evaluated.
Histologically, testis showed marked regressive changes in the seminiferous tubules in mice treated with nitrofurazone. Ten days after treatment, there was much depletion of germ cells in the seminiferous tubules, and the germinal epithelium was lined mainly with Sertoli cells, spermatogonia, spermatocytes, and a few round spermatids; intraepithelial vacuoles and multinucleated giant cells were also observed in tubules. By 20 days, regressive changes in the seminiferous tubules were further pronounced, and pachytene spermatocytes were the most advanced germ cells noticed in the tubules. In severe cases, the tubules were lined with a thin layer of Sertoli cells and spermatogonia. The treatment also caused marked reductions in the motility and number of spermatozoa in the cauda epididymidis, in weight and the level of fructose in the seminal vesicle, and in sialic acid level in the epididymis. Fifty six days after drug withdrawal, the alterations induced in the reproductive organs returned to control levels.
Our results suggest that nitrofurazone treatment in P mice induces marked alterations in the male reproductive organs, and that the alterations are reversible following cessation of treatment.
研究硝基呋喃衍生物5-硝基-2-糠醛缩氨基脲（呋喃西林）对帕克斯（P）品系小鼠雄性生殖器官的影响。
小鼠每天按64 mg/kg体重口服呋喃西林，持续10天和20天，并在最后一次治疗后24小时和/或56天处死。评估睾丸的组织学外观、附睾尾部精子的活力和数量，以及附睾和精囊的生化指标。
组织学上，用呋喃西林处理的小鼠睾丸生精小管出现明显的退行性变化。治疗10天后，生精小管中的生殖细胞大量减少，生精上皮主要由支持细胞、精原细胞、精母细胞和少数圆形精子细胞组成；小管内还观察到上皮内空泡和多核巨细胞。到20天时，生精小管的退行性变化进一步明显，粗线期精母细胞是小管中观察到的最成熟的生殖细胞。在严重的情况下，小管内衬有一层薄薄的支持细胞和精原细胞。该治疗还导致附睾尾部精子的活力和数量、精囊重量和果糖水平以及附睾唾液酸水平显著降低。停药56天后，生殖器官诱导的改变恢复到对照水平。
我们的结果表明，P小鼠接受呋喃西林治疗会导致雄性生殖器官出现明显改变，并且在停止治疗后这些改变是可逆的。
