Santagati F, Gerber J K, Blusch J H, Kokubu C, Peters H, Adamski J, Werner T, Balling R, Imai K
GSF-Research Center for Health and Environment, Institute of Mammalian Genetics, Neuherberg, Germany.
Mamm Genome. 2001 Mar;12(3):232-7. doi: 10.1007/s003350010267.
As a first step towards the identification of cis-regulatory elements of Pax9 by means of comparative genomics, we have analyzed genome regions encompassing the Pax9 gene in three vertebrate species, humans, mice (Mus musculus), and the Japanese pufferfish (Fugu rubripes). We show the genomic organization of Pax9 and its physical association with Nkx2-9 conserved in the three species. We discuss about possible implications of the conserved synteny between Pax9 and Nkx2-9 in a context of vertebrate evolution. This report also includes the first description of the primary structures of Fugu Pax9 and Nkx2-9. Furthermore, we report the identification of a novel upstream exon and putative transcription start sites in mouse Pax9. Our results suggest that transcription of Pax9 may be initiated at two alternative start sites and driven by TATA-less promoters.
作为通过比较基因组学鉴定Pax9顺式调控元件的第一步，我们分析了包括人类、小鼠（小家鼠）和日本河豚（红鳍东方鲀）这三种脊椎动物物种中Pax9基因的基因组区域。我们展示了Pax9的基因组组织及其与Nkx2 - 9在这三个物种中保守的物理关联。我们讨论了在脊椎动物进化背景下Pax9和Nkx2 - 9之间保守同线性的可能影响。本报告还首次描述了河豚Pax9和Nkx2 - 9的一级结构。此外，我们报告了在小鼠Pax9中鉴定出一个新的上游外显子和假定的转录起始位点。我们的结果表明，Pax9的转录可能在两个替代起始位点启动，并由无TATA框的启动子驱动。
