Delattre M, Briand S, Paces-Fessy M, Blanchet-Tournier M F
Institut Jacques Monod, Laboratoire Génétique du Développement et Evolution, Universités Pierre et Marie Curie et Denis Diderot, 2 place Jussieu, F-75251 Paris cedex 05, France.
Dev Genes Evol. 1999 May;209(5):294-300. doi: 10.1007/s004270050255.
The Suppressor of fused [Su(fu)] gene of Drosophila melanogaster encodes a protein containing a PEST sequence [sequence enriched in proline (P), glutamic acid (E), serine (S) and threonine (T)] which acts as an antagonist to the serine-threonine kinase Fused in Hedgehog (Hh) signal transduction during embryogenesis. The Su(fu) gene isolated from a distantly related Drosophila species, D. virilis, shows significantly high homology throughout its protein sequence with its D. melanogaster counterpart. We show that these two Drosophila homologs of Su(fu) are functionally interchangeable in enhancing the fused phenotype. We have also isolated mammalian homologs of Su(fu). The absence of the PEST sequence in the mammalian Su(fu) protein suggests a different regulation for this product between fly and vertebrates. Using the yeast two-hybrid method, we show that the murine Su(fu) protein can interact directly with the Fused and Cubitus interruptus proteins, known partners of Su(fu) in Drosophila. These data are discussed in the light of their evolutionary relationships.
黑腹果蝇的融合抑制基因[Su(fu)]编码一种含有PEST序列（富含脯氨酸（P）、谷氨酸（E）、丝氨酸（S）和苏氨酸（T）的序列）的蛋白质，该蛋白质在胚胎发育过程中作为刺猬信号通路（Hh）中丝氨酸-苏氨酸激酶Fused的拮抗剂发挥作用。从远缘果蝇物种果蝇中分离出的Su(fu)基因，在其整个蛋白质序列中与其黑腹果蝇对应物具有显著的高度同源性。我们发现，Su(fu)的这两个果蝇同源物在增强融合表型方面功能上是可互换的。我们还分离出了Su(fu)的哺乳动物同源物。哺乳动物Su(fu)蛋白中不存在PEST序列，这表明该产物在果蝇和脊椎动物之间存在不同的调控方式。利用酵母双杂交方法，我们发现小鼠Su(fu)蛋白可以直接与Fused和Cubitus interruptus蛋白相互作用，这两种蛋白是果蝇中已知的Su(fu)的相互作用伴侣。我们根据它们的进化关系对这些数据进行了讨论。
