从菠菜叶绿体中富集光系统I反应中心叶绿素。
Enrichment of photosystem I reaction center chlorophyll from spinach chloroplasts.
作者信息
Ikegami I, Kato S
出版信息
Biochim Biophys Acta. 1975 Mar 20;376(3):588-92. doi: 10.1016/0005-2728(75)90181-4.
PMID:1125224
Abstract
The reaction center chlorophyll of Photosystem I in spinach chloroplasts was highly enriched. Preparations having 5-9 chlorophylls per 1 P700 were obtained by treating the Photosystem I particles prepared by digitonin treatment of chloroplasts with wet diethyl ether. All P700 present in the extracted particles was found to be photoactive, undergoind oxidation upon illumination.
摘要
菠菜叶绿体中光系统I的反应中心叶绿素得到了高度富集。通过用湿乙醚处理经洋地黄皂苷处理叶绿体而制备的光系统I颗粒，获得了每1个P700含有5 - 9个叶绿素的制剂。发现提取颗粒中所有的P700都具有光活性，光照时会发生氧化反应。
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