Balaban N, Rasooly A
Department of Medical Pathology, University of California, Davis 95616, USA.
Int J Food Microbiol. 2001 Feb 28;64(1-2):33-40. doi: 10.1016/s0168-1605(00)00439-6.
Sample preparation is an important element in the detection of toxins in food samples. In this work, a simple analytical sample preparation method for recovery of small amount of staphylococcal enterotoxin B (SEB) and staphylococcal enterotoxin A (SEA) in food samples was developed. Cation exchanger carboxymethylcellulose (CM) was used for small-scale batch chromatography isolation of SEB from infant formula and from mushrooms spiked with SEB. The resulting materials were analyzed for SEB by Western immunoblotting. Nearly all of the extraneous substances in the sample were removed by this procedure with no significant loss of the toxin. Using this method, even small amounts of SE (0.75 ng/g) can be recovered and immunologically analyzed by Western blotting or by ELISA with a very low background. Because this method is effective, rapid, simple and inexpensive, it has the potential to be a general method for the preparation of samples used for analysis of SEs.
样品制备是食品样品中毒素检测的重要环节。在本研究中，开发了一种简单的分析样品制备方法，用于从食品样品中回收少量的葡萄球菌肠毒素B（SEB）和葡萄球菌肠毒素A（SEA）。阳离子交换剂羧甲基纤维素（CM）用于从婴儿配方奶粉和添加了SEB的蘑菇中进行小规模批量色谱分离SEB。通过蛋白质免疫印迹法对所得材料进行SEB分析。通过该程序，样品中几乎所有的杂质都被去除，毒素没有明显损失。使用该方法，即使是少量的SE（0.75 ng/g）也可以回收，并通过蛋白质免疫印迹法或酶联免疫吸附测定法进行免疫分析，背景非常低。由于该方法有效、快速、简单且成本低廉，它有可能成为一种用于制备用于SEs分析的样品的通用方法。
