苏云金芽孢杆菌外毒素对小鼠肝细胞核RNA生物合成及成熟的影响。

Effect of the exotoxin of Bacillus thuringiensis on the biosynthesis and maturation of mouse liver nuclear RNA.

作者信息

Mackedonski V V

出版信息

Biochim Biophys Acta. 1975 May 16;390(3):319-26. doi: 10.1016/0005-2787(75)90352-4.

Abstract

The effect of the exotoxin of Bacillus thuringiensis on the in vivo incorporation of [14-C] orotic acid into mouse liver nuclear rRNA and low molecular weight RNA was studied. The following results were obtained. 1. The exotoxin does not inhibit the synthesis of 45 S pre-rRNA, but causes a breakdown of these molecules. 2. The exotoxin inhibits the conversion of 38 S pre-rRNA into 32 S and 21 S. 3. The exotoxin inhibits the labelling of nuclear 5 S RNA, whereas the labelling of 4.6 S pre-tRNA is not affected. It is suggested that 5 S RNA may control the processing of 45 S pre-rRNA.

摘要

研究了苏云金芽孢杆菌外毒素对[14-C]乳清酸在小鼠肝细胞核rRNA和低分子量RNA体内掺入的影响。得到以下结果。1. 外毒素不抑制45S前体rRNA的合成，但会导致这些分子的降解。2. 外毒素抑制38S前体rRNA向32S和21S的转化。3. 外毒素抑制细胞核5S RNA的标记，而4.6S前体tRNA的标记不受影响。有人提出5S RNA可能控制45S前体rRNA的加工。