Honda K, Ishiko O, Wakasa K, Nishimura S, Tatsuta I, Ogita S
Department of Obstetrics and Gynecology, Osaka City University Medical School, 1-4-3, Asahimachi, Abeno-ku, Osaka 545-8585, Japan.
J Reprod Med. 2001 Feb;46(2):100-4.
To study the metachromatic stainability of the endocervical epithelium.
Surgical specimens that included endocervical epithelium were deparaffinized and stained with toluidine blue at various pH levels and after enzyme digestion. Glycosaminoglycans were separated from endocervical tissue and analyzed by electrophoresis.
The endocervical epithelium stained metachromatically purple with toluidine blue in pH 4.1 buffer, but the staining disappeared after chondroitinase digestion. The electrophoretic mobility of the glycosaminoglycan fraction separated from the endocervical epithelium was compatible with nonsulfated chondroitin.
Endocervical epithelium was identifiable from metachromatic staining below the internal os, and the electrophoretic results of the tissue extract suggested that nonsulfated chondroitin is the main component of glycosaminoglycans in endocervical epithelium.
研究宫颈管内膜上皮的异染性染色。
将包含宫颈管内膜上皮的手术标本脱石蜡处理，并在不同pH值水平下以及酶消化后用甲苯胺蓝染色。从宫颈管组织中分离出糖胺聚糖并通过电泳进行分析。
在pH 4.1缓冲液中，宫颈管内膜上皮用甲苯胺蓝染成异染性紫色，但在软骨素酶消化后染色消失。从宫颈管内膜上皮分离出的糖胺聚糖部分的电泳迁移率与非硫酸化软骨素相符。
宫颈管内膜上皮可通过宫颈内口以下的异染性染色来识别，并且组织提取物的电泳结果表明非硫酸化软骨素是宫颈管内膜上皮中糖胺聚糖的主要成分。
