Aparicio F, Sánchez-Navarro J A, Olsthoorn R C L, Pallás V, Bol J F
Instituto de Biología Molecular y Celular de Plantas, Universidad Politecnica de Valencia-CSIC, Avenida de los Naranjos s/n, 46022 Valencia, Spain1.
Institute of Molecular Plant Sciences, Gorlaeus Laboratories, Leiden University, PO Box 9502, 2300 RA Leiden, The Netherlands2.
J Gen Virol. 2001 Apr;82(Pt 4):947-951. doi: 10.1099/0022-1317-82-4-947.
Alfalfa mosaic virus (AMV) and Prunus necrotic ringspot virus (PNRSV) belong to the genera ALFAMOVIRUS: and ILARVIRUS:, respectively, of the family BROMOVIRIDAE: Initiation of infection by AMV and PNRSV requires binding of a few molecules of coat protein (CP) to the 3' termini of the inoculum RNAs and the CPs of the two viruses are interchangeable in this early step of the replication cycle. CIS:-acting sequences in PNRSV RNA 3 that are recognized by the AMV replicase were studied in in vitro replicase assays and by inoculation of AMV-PNRSV RNA 3 chimeras to tobacco plants and protoplasts transformed with the AMV replicase genes (P12 plants). The results showed that the AMV replicase recognized the promoter for minus-strand RNA synthesis in PNRSV RNA 3 but not the promoter for plus-strand RNA synthesis. A chimeric RNA with PNRSV movement protein and CP genes accumulated in tobacco, which is a non-host for PNRSV.
苜蓿花叶病毒（AMV）和李坏死环斑病毒（PNRSV）分别属于雀麦花叶病毒科的苜蓿花叶病毒属和等轴不稳环斑病毒属。AMV和PNRSV的感染起始需要少数衣壳蛋白（CP）分子与接种RNA的3'末端结合，并且在复制周期的这一早期步骤中，两种病毒的CP是可互换的。通过体外复制酶分析以及将AMV-PNRSV RNA 3嵌合体接种到烟草植株和用AMV复制酶基因转化的原生质体（P12植株）中，研究了PNRSV RNA 3中被AMV复制酶识别的顺式作用序列。结果表明，AMV复制酶识别PNRSV RNA 3中负链RNA合成的启动子，但不识别正链RNA合成的启动子。带有PNRSV移动蛋白和CP基因的嵌合RNA在烟草中积累，烟草是PNRSV的非寄主植物。
