Onuoha G N, Alpar E K
Department of Surgery, The University of Birmingham, Birmingham, UK.
Regul Pept. 2001 May 5;99(1):17-20. doi: 10.1016/s0167-0115(01)00216-6.
To determine whether concentrations of the N-terminal peptide of pro-atrial natriuretic peptide (proANP) and of alpha atrial natriuretic peptide 1-28 (aANP) releases are affected by myoskeletal injuries, samples from 24 patients with muscle injuries were therefore collected within 48 h of injury. The mean age of patients was 65; range: 17-90 years. These were compared with 18 non-injured subjects with a mean age of 40; range: 17-80 years. A specific enzyme immunoassay (EIA) method suitable for the determination of proANP and aANP was used. aANP required plasma extraction and no extraction was needed for proANP determination.ProANP level was significantly higher in patients on admission and this level was maintained 24 h after admission (p < 0.05) compared to controls. However, aANP 1-28 level remained statistically unchanged in the patients samples. The level of proANP was over 10 times greater than the levels obtained with aANP. N-terminal peptide of proANP may be a supplementary tool in the study of early phase of myoskeletal injuries in human.
为了确定肌骨骼损伤是否会影响前心钠素（proANP）的N端肽浓度以及α心钠素1-28（aANP）的释放，因此在损伤后48小时内收集了24例肌肉损伤患者的样本。患者的平均年龄为65岁；范围：17-90岁。将这些患者与18名平均年龄为40岁的非损伤受试者进行比较；范围：17-80岁。使用了一种适用于测定proANP和aANP的特异性酶免疫测定（EIA）方法。aANP需要血浆提取，而proANP测定则无需提取。与对照组相比，患者入院时proANP水平显著更高，且入院后24小时该水平维持不变（p<0.05）。然而，患者样本中的aANP 1-28水平在统计学上保持不变。proANP的水平比aANP的水平高出10倍以上。proANP的N端肽可能是研究人类肌骨骼损伤早期阶段的一种辅助工具。
