Anaya-Martínez V, Montiel-Flores E, Espinosa-Villanueva J, García-Hernández F
Laboratorio de Neuromorfología, Departamento de Neurociencias, Escuela Nacional de Estudios Profesionales (ENEP) Iztacala, Universidad Nacional Autónoma de México (UNAM), Mexico City, Mexico.
Arch Med Res. 2000 Nov-Dec;31(6):551-7. doi: 10.1016/s0188-4409(00)00247-2.
Because of their lack of long-term viability, adrenal tissue transplants have shown limited success in alleviating the motor disturbances associated with experimental and pathologic striatal dopamine denervation. In this study, we examined how the graft placement site influences adrenal medulla transplant survival and its relation with the reduction of motor deficits in rats bearing unilateral 6-OHDA lesion.
One or 5 microL of fetal adrenal medullar tissue was grafted either inside the striatal parenchyma or into the lateral ventricle in contact with the dopamine-denervated striatum. Motor disturbances, as assessed by apomorphine-induced rotation, were correlated to the graft morphologic survival features.
Apomorphine-induced rotation showed a marginal reduction of 11% in all groups independently of graft survival features or placement site. Intrastriatal transplants showed limited viability characterized by a substantial loss of graft initial volume as well as fewer and smaller chromaffin cells compared to ventricular grafts, which had a reduced loss of graft initial volume and more and larger chromaffin cells.
Although the lateral ventricle may favor adrenal medulla transplant viability, their induced motor outcome is comparable to that induced by less viable intrastriatal grafts, suggesting that the implanted dopamine-producing cells may interact and influence striatal neurons better when placed in close proximity.
由于肾上腺组织移植缺乏长期生存能力，其在缓解与实验性和病理性纹状体多巴胺去神经支配相关的运动障碍方面成效有限。在本研究中，我们探讨了移植部位如何影响肾上腺髓质移植的存活及其与单侧6-羟基多巴胺（6-OHDA）损伤大鼠运动功能缺损减轻之间的关系。
将1或5微升胎儿肾上腺髓质组织移植到纹状实质内或与多巴胺去神经支配的纹状体接触的侧脑室内。通过阿扑吗啡诱导的旋转评估的运动障碍与移植的形态学存活特征相关。
无论移植存活特征或移植部位如何，所有组中阿扑吗啡诱导的旋转均显示出11%的轻微降低。与脑室移植相比，纹状体内移植显示出有限的生存能力，其特征为移植初始体积大量减少以及嗜铬细胞数量减少且体积变小，而脑室移植的移植初始体积损失减少，嗜铬细胞数量更多且体积更大。
尽管侧脑室可能有利于肾上腺髓质移植的存活，但其诱导的运动结果与生存能力较差的纹状体内移植所诱导的结果相当，这表明植入的产生多巴胺的细胞在近距离放置时可能更好地与纹状体神经元相互作用并对其产生影响。
