Kompa A R, Summers R J
Department of Pharmacology, Monash University, Victoria 3800, Australia.
J Pharmacol Toxicol Methods. 2000 May-Jun;43(3):199-203. doi: 10.1016/s1056-8719(00)00103-9.
Coronary artery ligation in the rat provides a useful experimental model of cardiac failure; however, this procedure carries with it a high mortality rate (50%). In this study, we used lidocaine (10 mg/kg, i.m.) before coronary artery ligation and 2 h after surgery to minimise the incidence of ventricular fibrillation (VF) that leads to sudden death in this model. We found that coronary artery ligation, using lidocaine in conjunction with a modified surgical procedure, had a mortality rate of 15%, much lower than reported in previous studies using this model. These modifications allow for the production of larger infarcts with 29% of animals having an infarct size > 50% of the epicardial surface. Infarct size in our myocardial infarction (MI) group varied between 5% and 75% of the left ventricular (LV) surface area resulting in a mean infarct size of 41.3 +/- 1.3% for the epicardial surface and 40.0 +/- 1.3% for the endocardial surface.
大鼠冠状动脉结扎术提供了一种有用的心力衰竭实验模型；然而，该手术伴随着较高的死亡率（50%）。在本研究中，我们在冠状动脉结扎前及术后2小时使用利多卡因（10mg/kg，肌肉注射），以降低该模型中导致猝死的室颤（VF）发生率。我们发现，联合使用利多卡因及改良手术方法进行冠状动脉结扎，死亡率为15%，远低于此前使用该模型的研究报告。这些改良方法能够产生更大的梗死灶，29%的动物梗死灶大小超过心外膜表面积的50%。我们心肌梗死（MI）组的梗死灶大小在左心室（LV）表面积的5%至75%之间变化，心外膜表面积的平均梗死灶大小为41.3±1.3%，心内膜表面积为40.0±1.3%。
