Rickert W S, McBride-Warren P A
Can J Biochem. 1975 Mar;53(3):269-74. doi: 10.1139/o75-038.
The acid protease produced by a strain of Mucor miehei isolated in Cuba was purified by column electrofocusing and partially characterized as to amino-acid composition, molecular weight, helical content, total carbohydrate content, and approximate isoelectric point; A detailed comparison of these results was reported previously for Mucor miehei protease (Ottesen, M. & Rickert, W;S. (1970) C.R. Trav. Labmcarlsberg 37, 301) suggested that the two enzymes are similar but not identicalmthis conclusion was reinforced by an analysis of circular-dichroism spectra.
对从古巴分离出的一株米黑根霉所产生的酸性蛋白酶,通过柱电聚焦法进行了纯化,并对其氨基酸组成、分子量、螺旋含量、总碳水化合物含量及近似等电点进行了部分表征;先前已报道了米黑根霉蛋白酶的这些结果的详细比较情况(奥特森,M. 及里克特,W.S.(1970年)《嘉士伯实验室研究报告》37卷,第301页),结果表明这两种酶相似但并不相同,圆二色光谱分析进一步证实了这一结论。