Kushwaha S C, Kates M, Martin W G
Can J Biochem. 1975 Mar;53(3):284-92. doi: 10.1139/o75-040.
The purple membrance (bacteriorhodopsin) isolated from cells of Halobacterium cutirubrum grown anaerobically in the light was shown to contain 77% protein and 20% lipils by weight. The protein component consisted of a single protein moeity, having a molecular weight of (19.6 plus or minus 0.8) times 10-3, complexed with retinal in mole ratio of 2:1, respectively. The protein moeity is not glycosylated but may be phosphorylated (ca. 2 mol of phosphate per mole of protein). The red membrance contains 56%protein and 38% lipids, including bacterioruberins. Several polypeptide components are present including some which may be glycosylated and/or phosphorylated. The lipids of both membranes contained phosphatidyl glycerophosphate (52%) and phosphatidyl glycerol (3-4%) but the sulfated lipid components, glycolipid sulfate and phosphatidyl glycerosulfate, were present exclusively in the purple membrane, the red membrane containing instead two unidentified glycolipids. Neutral lipids (squalenes, vitamin 75-8, etc.) were present in both membranes to the extent of 7-9%.
从在光照下厌氧生长的盐生盐杆菌细胞中分离出的紫色膜（细菌视紫红质）经重量分析表明，其含有77%的蛋白质和20%的脂质。蛋白质成分由单一蛋白质部分组成，分子量为(19.6±0.8)×10⁻³，与视黄醛的摩尔比为2:1。该蛋白质部分未糖基化，但可能磷酸化（每摩尔蛋白质约2摩尔磷酸盐）。红色膜含有56%的蛋白质和38%的脂质，包括细菌红素。存在几种多肽成分，其中一些可能糖基化和/或磷酸化。两种膜的脂质都含有磷脂酰甘油磷酸（52%）和磷脂酰甘油（3 - 4%），但硫酸化脂质成分，糖脂硫酸盐和磷脂酰甘油硫酸盐，仅存在于紫色膜中，红色膜则含有两种未鉴定的糖脂。中性脂质（角鲨烯、维生素75 - 8等）在两种膜中的含量均为7 - 9%。
