猫肝细胞生长因子（HGF）cDNA的分子克隆

Molecular cloning of feline hepatocyte growth factor (HGF) cDNA.

作者信息

Kobayashi Y, Nakamura N, Ishizaka T, Masuda K, Ohno K, Tsujimoto H

机构信息

Department of Veterinary Internal Medicine, Faculty of Agriculture, The University of Tokyo, Japan.

出版信息

J Vet Med Sci. 2001 Feb;63(2):211-4. doi: 10.1292/jvms.63.211.

DOI:10.1292/jvms.63.211

PMID:11258464

Abstract

Hepatocyte growth factor (HGF) is a pleiotropic cytokine responsible for regeneration, development and maintenance of various organs, and growth, invasion and metastasis of tumor cells. A full-length feline HGF cDNA was cloned and sequenced by RT-PCR from cat liver. Feline HGF consists of 728 amino acid and contains alpha- and beta-chains encoded in a single open reading frame. The predicted amino acid sequence of feline HGF showed 93.2, 93.3 and 93.3% homology with those of human, mouse and rat HGF, respectively. The putative proteolytic processing site, all cysteine residues, and four potential glycosylation sites are conserved in all species. Therefore, feline HGF is expected to have a similar three-dimensional structure to human, mouse and rat HGF.

摘要

肝细胞生长因子（HGF）是一种多效性细胞因子，负责各种器官的再生、发育和维持，以及肿瘤细胞的生长、侵袭和转移。通过逆转录聚合酶链反应（RT-PCR）从猫肝脏中克隆并测序了全长猫HGF cDNA。猫HGF由728个氨基酸组成，包含在单个开放阅读框中编码的α链和β链。猫HGF的预测氨基酸序列与人类、小鼠和大鼠HGF的氨基酸序列分别具有93.2%、93.3%和93.3%的同源性。假定的蛋白水解加工位点、所有半胱氨酸残基和四个潜在的糖基化位点在所有物种中都是保守的。因此，预计猫HGF具有与人类、小鼠和大鼠HGF相似的三维结构。