Tobi M, Chintalapani S, Kithier K, Clapp N
Department of Medicine, Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, Michigan, USA.
Dig Dis Sci. 2000 Dec;45(12):2290-7. doi: 10.1023/a:1005622521294.
As an animal model for human inflammatory bowel disease and colorectal cancer, the cotton-top tamarin remains controversial. Demonstration of antigenic similarity to the human would enhance its validity. Using colonic extracts and washings, we compared binding of seven monoclonal antibodies reactive with bowel and cancer antigens in both tamarins and humans with inflammatory bowel disease. Additionally, telomerase activity was tested for. Expression of a mucin antigen specific to human cancer was increased in tamarin colonic washings as well as aminoproteoglycans and EGFR in tamarin extracts, as compared to those of humans with inflammatory bowel disease (P < 0.005). An adenoma-associated antigen and k-ras p21 protein were negative in the tamarin. A trend to greater telomerase activity exists in tamarins. The antigenic similarity validates this model for human inflammatory bowel disease and colorectal cancer. A trend to increased telomerase activity in tamarins is consistent with the greater predisposition to cancer in these animals.
作为人类炎症性肠病和结直肠癌的动物模型，棉顶狨猴仍然存在争议。证明其与人类的抗原相似性将提高其有效性。我们使用结肠提取物和冲洗液，比较了七种与肠道和癌症抗原反应的单克隆抗体在狨猴和患有炎症性肠病的人类中的结合情况。此外，还检测了端粒酶活性。与患有炎症性肠病的人类相比，狨猴结肠冲洗液中人类癌症特异性粘蛋白抗原的表达增加，狨猴提取物中的氨基蛋白聚糖和表皮生长因子受体也增加（P < 0.005）。腺瘤相关抗原和k-ras p21蛋白在狨猴中呈阴性。狨猴存在端粒酶活性增强的趋势。抗原相似性验证了该模型可用于人类炎症性肠病和结直肠癌的研究。狨猴中端粒酶活性增加的趋势与这些动物更高的癌症易感性一致。
