Liu Y, Bergan R
Department of Medicine, Northwestern University Medical School, and the Lurie Cancer Center of Northwestern University, Chicago, IL 60611-3008, USA.
Antisense Nucleic Acid Drug Dev. 2001 Feb;11(1):7-14. doi: 10.1089/108729001750072083.
Prior studies have shown that electroporation is a simple and effective method for the introduction of oligonucleotides (ODN) into cells. In ex vivo bone marrow purging models, electroporation of ODN into cells has been associated with selective killing of human neoplastic cells while sparing hematopoietic stem cells. Prior studies used conventional electroporation methods (i.e., exponential decay) to introduce ODN into cells. Square wave electroporation allows the delivery of a more defined and regulated electrical pulse and is associated with high transfection efficiencies in a variety of systems. The current study was undertaken to determine whether square wave electroporation was more effective than exponential decay electroporation for the delivery of ODN into hematopoietic cells. Using fluorescein-tagged ODN and K562, chronic myelogenous leukemia (CML) cells, higher transfection rates were observed after square wave electroporation. In addition, c-myc antisense ODN were more effective in reducing c-myc protein when introduced by square wave electroporation, as compared with introduction by exponential decay electroporation. Square wave electroporation is thus identified as the optimal method for delivering ODN into hematopoietic cells.
先前的研究表明，电穿孔是一种将寡核苷酸（ODN）导入细胞的简单有效方法。在体外骨髓净化模型中，将ODN电穿孔导入细胞与选择性杀伤人类肿瘤细胞同时保留造血干细胞有关。先前的研究使用传统的电穿孔方法（即指数衰减）将ODN导入细胞。方波电穿孔能够传递更明确和可控的电脉冲，并且在各种系统中都具有高转染效率。当前的研究旨在确定方波电穿孔在将ODN导入造血细胞方面是否比指数衰减电穿孔更有效。使用荧光素标记的ODN和慢性髓性白血病（CML）细胞系K562，方波电穿孔后观察到更高的转染率。此外，与通过指数衰减电穿孔导入相比，通过方波电穿孔导入c-myc反义ODN在降低c-myc蛋白方面更有效。因此，方波电穿孔被确定为将ODN导入造血细胞的最佳方法。
