Lindner R
Department of Cell Biology, Center of Anatomy Hannover Medical School, Germany.
Electrophoresis. 2001 Feb;22(3):386-93. doi: 10.1002/1522-2683(200102)22:3<386::AID-ELPS386>3.0.CO;2-T.
Many different methods for the fractionation of subcellular organelles have been reported. However, no protocol for rapid separation of plasma membrane, Golgi/trans-Golgi network (TGN) and endosomes is available to date. Such a method is a prerequisite for a quantitative biochemical analysis of vesicular transport from the Golgi/TGN compartment to plasma membrane and endosomes. Here a density gradient electrophoresis protocol is described that allows the fractionation of these organelles in one step. This protocol requires only low-cost instrumentation available in most biochemical laboratories.
已经报道了许多用于亚细胞器分级分离的不同方法。然而，迄今为止，尚无用于快速分离质膜、高尔基体/反式高尔基体网络（TGN）和内体的方案。这样一种方法是对从高尔基体/TGN区室到质膜和内体的囊泡运输进行定量生化分析的先决条件。本文描述了一种密度梯度电泳方案，该方案可在一步中对这些细胞器进行分级分离。该方案仅需要大多数生化实验室都具备的低成本仪器。
