Picard C, Wysocki J, Linard C, Garcia-Villar R, Bueno L, Griffiths N M, Fioramonti J
Institut de Protection et de Sureté Nucléaire, Digestive Radiobiology Unit, Fontenay-aux-Roses, France.
Int J Radiat Biol. 2001 Mar;77(3):349-56. doi: 10.1080/09553000010002889.
To assess the early effects of primary afferent nerve suppression by systemic treatment with the neurotoxin capsaicin in an acute model of abdominal irradiation in rats (10Gy, gamma).
Changes in myeloperoxidase (MPO) activity, calcitonin gene-related peptide (CGRP) tissue content, number of mast cells and apoptotic cells were determined in jejunum and ileum in four groups of rat male Wistar (vehicle sham-irradiated, vehicle irradiated, capsaicin sham-irradiated and capsaicin irradiated) at 1 and 3 days post-irradiation.
In vehicle irradiated rats, CGRP was significantly increased from the first day after irradiation in jejunal mucosa; MPO activity increased in both segments at day 3 but not at day 1 after irradiation; the number of detectable mucosal mast cells dropped to nearly zero on days 1 and 3, while the apoptotic cells in the intestinal mucosa were significantly increased at day 1. Similar results were obtained for mast cells and apoptosis in capsaicin irradiated rats as compared to capsaicin sham-irradiated rats, while MPO activity was significantly increased and CGRP concentration in jejunal mucosa significantly decreased from the first day in these rats in comparison with capsaicin sham-irradiated rats.
Intestinal sensory innervation seems not to have a major protective role against a radiation-induced intestinal inflammatory reaction.
在大鼠腹部急性照射（10Gy，γ射线）模型中，评估通过全身给予神经毒素辣椒素抑制初级传入神经的早期效应。
在照射后1天和3天，测定四组雄性Wistar大鼠（假照射对照组、照射对照组、假照射辣椒素组、照射辣椒素组）空肠和回肠中髓过氧化物酶（MPO）活性、降钙素基因相关肽（CGRP）组织含量、肥大细胞数量和凋亡细胞数量的变化。
在照射对照组大鼠中，空肠黏膜中CGRP从照射后第一天起显著增加；照射后第3天，两段肠管的MPO活性均升高，但第1天未升高；第1天和第3天，可检测到的黏膜肥大细胞数量降至几乎为零，而肠黏膜中的凋亡细胞在第1天显著增加。与假照射辣椒素组大鼠相比，照射辣椒素组大鼠的肥大细胞和凋亡情况得到类似结果，而与假照射辣椒素组大鼠相比，这些大鼠空肠黏膜中的MPO活性从第一天起显著升高，CGRP浓度显著降低。
肠道感觉神经支配似乎对辐射诱导的肠道炎症反应没有主要的保护作用。
