Smith G C, Wu W X, Nathanielsz P W
Laboratory for Pregnancy and Newborn Research, Department of Physiology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA.
Biol Reprod. 2001 Apr;64(4):1131-7. doi: 10.1095/biolreprod64.4.1131.
We determined the effect of gestational age and labor on the regional expression of prostanoid receptor genes in baboon myometrium. Cesarean hysterectomy was performed on 15 pregnant baboons of known gestational age in the last third of pregnancy, five of them during spontaneous term labor. Expression of prostanoid receptor genes was studied using Northern blot analysis. Transcripts of similar size to the human were detected for prostanoid EP(1), EP(2), EP(3), EP(4), IP, FP, and TP receptor genes using Northern blot analysis. There were no gestational age-related changes in expression of these genes. Expression of EP(1), EP(3), and IP receptor RNA mRNA was significantly higher in myometrium from the fundus (compared with the lower segment), whereas EP(2) gene expression was significantly lower in the fundus. Labor was associated with a reduction in the regional variation of both EP(2) and IP receptor gene expression, but not EP(1) and EP(3) expression. Labor was also associated with an overall lower level of expression of EP(2) receptor mRNA. We conclude that regional and labor-related variation in myometrial expression of prostanoid receptor genes may have a key role in primate parturition.
我们确定了胎龄和分娩对狒狒子宫肌层中前列腺素受体基因区域表达的影响。在妊娠晚期，对15只已知胎龄的怀孕狒狒实施剖宫产子宫切除术，其中5只处于足月自然分娩期。使用Northern印迹分析法研究前列腺素受体基因的表达。通过Northern印迹分析法检测到前列腺素EP(1)、EP(2)、EP(3)、EP(4)、IP、FP和TP受体基因与人类大小相似的转录本。这些基因的表达不存在与胎龄相关的变化。子宫底肌层中EP(1)、EP(3)和IP受体RNA mRNA的表达显著高于（与下段相比）子宫下段，而EP(2)基因在子宫底的表达显著较低。分娩与EP(2)和IP受体基因表达的区域差异减小有关，但与EP(1)和EP(3)表达无关。分娩还与EP(2)受体mRNA的总体表达水平较低有关。我们得出结论，前列腺素受体基因在子宫肌层中的区域和分娩相关差异可能在灵长类动物分娩中起关键作用。
